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Plasmid Files

CLIP-tag™

Human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylcytosine derivatives.

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CLIP-tag Sequence and MapCLIP-tag.dna
Map and Sequence File   
Sequence Author:  New England Biolabs
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 500 400 300 200 100 CLIP-tag™ End (546) BssS α I (511) ApaI (470) PspOMI (466) BmgBI (462) EaeI * (350) BseRI (244) Bsu36I (218) EciI (176) BtgI (128) BsaHI (125) BglI (47) Start (0) CLIP-tag™ 546 bp
End  (546)
0 sites
BssSαI  (511)
1 site
C A C G A G G T G C T C
ApaI  (470)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (466)
1 site
G G G C C C C C C G G G
BmgBI  (462)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
EaeI  (350)
1 site
Y G G C C R R C C G G Y
* Blocked by Dcm methylation.
BseRI  (244)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Bsu36I  (218)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EciI  (176)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BtgI  (128)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaHI  (125)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BglI  (47)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
Start  (0)
0 sites
CLIP-tag™
1 .. 546  =  546 bp
182 amino acids  =  19.4 kDa
Product: human
O6-alkylguanine-DNA-alkyltransferase variant that
forms covalent bonds with benzylcytosine
derivatives
fast-reacting variant, optimized for mammalian
expression
CLIP-tag™
1 .. 546  =  546 bp
182 amino acids  =  19.4 kDa
Product: human
O6-alkylguanine-DNA-alkyltransferase variant that
forms covalent bonds with benzylcytosine
derivatives
fast-reacting variant, optimized for mammalian
expression
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