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Plasmid Files

GST

Glutathione S-transferase from Schistosoma japonicum.

 
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 600 400 200 GST End (657) ScaI (574) TatI (572) BclI * (435) SwaI (428) BstBI (398) XmnI (394) BsrBI (292) BsgI (287) NspI (243) AflIII - PciI (239) TaqII (235) MscI (208) EaeI (206) BtgZI (119) MslI (95) BspQI - EarI - SapI (80) EcoO109I (33) EcoNI (11) Start (0) GST 657 bp
End  (657)
0 sites
ScaI  (574)
1 site
A G T A C T T C A T G A
TatI  (572)
1 site
W G T A C W W C A T G W
BclI  (435)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SwaI  (428)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BstBI  (398)
1 site
T T C G A A A A G C T T
XmnI  (394)
1 site
G A A N N N N T T C C T T N N N N A A G
BsrBI  (292)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
BsgI  (287)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NspI  (243)
1 site
R C A T G Y Y G T A C R
AflIII  (239)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (239)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
TaqII  (235)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
MscI  (208)
1 site
T G G C C A A C C G G T
EaeI  (206)
1 site
Y G G C C R R C C G G Y
BtgZI  (119)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MslI  (95)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BspQI  (80)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (80)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (80)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
EcoO109I  (33)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
EcoNI  (11)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Start  (0)
0 sites
GST
1 .. 657  =  657 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from
Schistosoma japonicum
GST
1 .. 657  =  657 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from
Schistosoma japonicum
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