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Plasmid Files

HaloTag®

Modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives.

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HaloTag Sequence and MapHaloTag.dna
Map and Sequence File   
Sequence Author:  Promega
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 800 600 400 200 HaloTag® End (891) PaeR7I - SmlI - XhoI (877) AccI (871) SalI (870) BssHII (859) BbsI (833) SmaI (806) TspMI - XmaI (804) BaeGI - Bme1580I (724) SgrAI (634) HaeII (612) BglI (606) AcuI (566) PshAI (547) MspA1I (540) TaqII (514) AclI (497) BspDI * - ClaI * (488) BclI * (482) AatII (470) ZraI (468) BsaHI (467) StuI * (451) BsaI (433) ApoI (427) BanII (325) EarI (287) PasI (282) BmgBI (252) DrdI - PflFI - Tth111I (250) MmeI (239) BsrDI (182) BpmI (173) BsaAI (138) AflIII (137) BseRI (120) BstEII (118) AleI - MslI (100) SphI (66) PflMI (42) Start (0) HaloTag® 891 bp
End  (891)
0 sites
PaeR7I  (877)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
SmlI  (877)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
XhoI  (877)
1 site
C T C G A G G A G C T C
AccI  (871)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (870)
1 site
G T C G A C C A G C T G
BssHII  (859)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BbsI  (833)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SmaI  (806)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (804)
1 site
C C C G G G G G G C C C
XmaI  (804)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BaeGI  (724)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (724)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
SgrAI  (634)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
HaeII  (612)
1 site
R G C G C Y Y C G C G R
BglI  (606)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AcuI  (566)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PshAI  (547)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
MspA1I  (540)
1 site
C M G C K G G K C G M C
TaqII  (514)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
AclI  (497)
1 site
A A C G T T T T G C A A
BspDI  (488)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (488)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BclI  (482)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AatII  (470)
1 site
G A C G T C C T G C A G
ZraI  (468)
1 site
G A C G T C C T G C A G
BsaHI  (467)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
StuI  (451)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BsaI  (433)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ApoI  (427)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BanII  (325)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
EarI  (287)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
PasI  (282)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BmgBI  (252)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
DrdI  (250)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PflFI  (250)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (250)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MmeI  (239)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrDI  (182)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BpmI  (173)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaAI  (138)
1 site
Y A C G T R R T G C A Y
AflIII  (137)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BseRI  (120)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BstEII  (118)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AleI  (100)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (100)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SphI  (66)
1 site
G C A T G C C G T A C G
PflMI  (42)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Start  (0)
0 sites
HaloTag®
1 .. 891  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives
HaloTag®
1 .. 891  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives
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