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Plasmid Files

M13mp18

M13 bacteriophage vector containing a multiple cloning site (MCS). The MCS is reversed relative to M13mp19.

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M13mp18 Sequence and MapM13mp18.dna
Map and Sequence File   
Sequence Author:  New England Biolabs
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 BglII (6934) SwaI (6785) AleI (6576) Bsu36I (6508) BglI (6436) FspI (6426) PvuI (6407) BmrI (6326) HindIII (6281) SphI (6279) PstI - SbfI (6273) HincII (6265) AccI (6264) SalI (6263) XbaI (6257) BamHI (6251) SmaI (6248) KpnI - TspMI - XmaI (6246) Acc65I (6242) SacI (6240) Eco53kI (6238) EcoRI (6230) lac operator Bts α I (6094) PluTI (6004) SfoI (6002) BsaHI - NarI * (6001) KasI (6000) BsmBI (5976) DrdI (5764) DraIII (5720) NaeI (5614) BsrFI - NgoMIV (5612) MscI (5081) PacI (4135) BsrGI (1021) SnaBI (1270) BspHI (1299) BsmI (1751) BseRI (2022) BaeGI - Bme1580I (2091) AlwNI (2191) AfeI (3040) M13mp18 7249 bp
BglII  (6934)
1 site
A G A T C T T C T A G A
SwaI  (6785)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AleI  (6576)
1 site
C A C N N N N G T G G T G N N N N C A C
Bsu36I  (6508)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BglI  (6436)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (6426)
1 site
T G C G C A A C G C G T
PvuI  (6407)
1 site
C G A T C G G C T A G C
BmrI  (6326)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
HindIII  (6281)
1 site
A A G C T T T T C G A A
SphI  (6279)
1 site
G C A T G C C G T A C G
PstI  (6273)
1 site
C T G C A G G A C G T C
SbfI  (6273)
1 site
C C T G C A G G G G A C G T C C
HincII  (6265)
1 site
G T Y R A C C A R Y T G
AccI  (6264)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (6263)
1 site
G T C G A C C A G C T G
XbaI  (6257)
1 site
T C T A G A A G A T C T
BamHI  (6251)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SmaI  (6248)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (6246)
1 site
G G T A C C C C A T G G
TspMI  (6246)
1 site
C C C G G G G G G C C C
XmaI  (6246)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (6242)
1 site
G G T A C C C C A T G G
SacI  (6240)
1 site
G A G C T C C T C G A G
Eco53kI  (6238)
1 site
G A G C T C C T C G A G
EcoRI  (6230)
1 site
G A A T T C C T T A A G
BtsαI  (6094)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
PluTI  (6004)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (6002)
1 site
G G C G C C C C G C G G
BsaHI  (6001)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (6001)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (6000)
1 site
G G C G C C C C G C G G
BsmBI  (5976)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
DrdI  (5764)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
DraIII  (5720)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (5614)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BsrFI  (5612)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
NgoMIV  (5612)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
MscI  (5081)
1 site
T G G C C A A C C G G T
PacI  (4135)
1 site
T T A A T T A A A A T T A A T T
BsrGI  (1021)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SnaBI  (1270)
1 site
T A C G T A A T G C A T
BspHI  (1299)
1 site
T C A T G A A G T A C T
BsmI  (1751)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BseRI  (2022)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BaeGI  (2091)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (2091)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
AlwNI  (2191)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AfeI  (3040)
1 site
A G C G C T T C G C G A
M13 gene IV
4219 .. 5499  =  1281 bp
426 amino acids  =  45.9 kDa
Product: pIV
phage assembly
M13 gene IV
4219 .. 5499  =  1281 bp
426 amino acids  =  45.9 kDa
Product: pIV
phage assembly
M13 gene III
1578 .. 2852  =  1275 bp
424 amino acids  =  44.6 kDa
Product: pIII
minor coat protein
M13 gene III
1578 .. 2852  =  1275 bp
424 amino acids  =  44.6 kDa
Product: pIII
minor coat protein
M13 gene II
6848 .. 831  =  1233 bp
410 amino acids  =  46.2 kDa
Product: pII
replication
M13 gene II
6848 .. 831  =  1233 bp
410 amino acids  =  46.2 kDa
Product: pII
replication
lacZα
6216 .. 6722  =  507 bp
168 amino acids  =  18.9 kDa
Product: LacZα fragment of β-galactosidase
lacZα
6216 .. 6722  =  507 bp
168 amino acids  =  18.9 kDa
Product: LacZα fragment of β-galactosidase
M13 gene VI
2855 .. 3193  =  339 bp
112 amino acids  =  12.4 kDa
Product: pVI
minor coat protein
M13 gene VI
2855 .. 3193  =  339 bp
112 amino acids  =  12.4 kDa
Product: pVI
minor coat protein
M13 gene V
843 .. 1106  =  264 bp
87 amino acids  =  9.7 kDa
Product: pV
replication
M13 gene V
843 .. 1106  =  264 bp
87 amino acids  =  9.7 kDa
Product: pV
replication
M13 gene VIII
1301 .. 1522  =  222 bp
73 amino acids  =  7.6 kDa
Product: pVIII
major coat protein
M13 gene VIII
1301 .. 1522  =  222 bp
73 amino acids  =  7.6 kDa
Product: pVIII
major coat protein
M13 gene VII
1108 .. 1209  =  102 bp
33 amino acids  =  3.6 kDa
Product: pVII
minor coat protein
M13 gene VII
1108 .. 1209  =  102 bp
33 amino acids  =  3.6 kDa
Product: pVII
minor coat protein
lac promoter
6142 .. 6172  =  31 bp
   Segment 1:  -35  
   6142 .. 6147  =  6 bp
promoter for the E. coli lac operon
lac promoter
6142 .. 6172  =  31 bp
   Segment 2:  
   6148 .. 6165  =  18 bp
promoter for the E. coli lac operon
lac promoter
6142 .. 6172  =  31 bp
   Segment 3:  -10  
   6166 .. 6172  =  7 bp
promoter for the E. coli lac operon
lac promoter
6142 .. 6172  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
6180 .. 6196  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
6180 .. 6196  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 gene I
3195 .. 4241  =  1047 bp
348 amino acids  =  39.6 kDa
Product: pI
phage assembly
M13 gene I
3195 .. 4241  =  1047 bp
348 amino acids  =  39.6 kDa
Product: pI
phage assembly
M13 ori
5487 .. 5867  =  381 bp
M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
M13 ori
5487 .. 5867  =  381 bp
M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
M13 gene X
496 .. 831  =  336 bp
111 amino acids  =  12.7 kDa
Product: pX
replication
M13 gene X
496 .. 831  =  336 bp
111 amino acids  =  12.7 kDa
Product: pX
replication
M13 gene IX
1206 .. 1304  =  99 bp
32 amino acids  =  3.7 kDa
Product: pIX
minor coat protein
M13 gene IX
1206 .. 1304  =  99 bp
32 amino acids  =  3.7 kDa
Product: pIX
minor coat protein
MCS
6230 .. 6286  =  57 bp
multiple cloning site
MCS
6230 .. 6286  =  57 bp
multiple cloning site
M13 rev
6204 .. 6220  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
6204 .. 6220  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
6290 .. 6306  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
6290 .. 6306  =  17 bp
common sequencing primer, one of multiple similar
variants
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