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Plasmid Files

MBP

Maltose binding protein from E. coli.

 
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 1000 750 500 250 sign... MBP End (1176) BclI * (1118) AfeI (986) BanI - PspFI (976) BseYI (972) TaqII * (964) BlpI (862) MmeI (800) BtgI (763) BsaBI (751) AlwNI - BstAPI (721) BsmI (696) MslI (689) BmgBI (618) EaeI * (550) BssHII (512) BstXI (461) BglII (435) PsiI (428) PvuI (401) BsiWI (366) BciVI (356) PfoI * (332) TsoI (272) BspEI (195) BsaI (139) EciI (51) NmeAIII (47) BsiHKAI (21) ApaLI (17) Start (0) MBP 1176 bp
End  (1176)
0 sites
BclI  (1118)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AfeI  (986)
1 site
A G C G C T T C G C G A
BanI  (976)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
PspFI  (976)
1 site
C C C A G C G G G T C G
BseYI  (972)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
TaqII  (964)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9
* Blocked by EcoKI methylation.
Sticky ends from different TaqII sites may not be compatible.
BlpI  (862)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
MmeI  (800)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgI  (763)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaBI  (751)
1 site
G A T N N N N A T C C T A N N N N T A G
AlwNI  (721)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstAPI  (721)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BsmI  (696)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
MslI  (689)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmgBI  (618)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
EaeI  (550)
1 site
Y G G C C R R C C G G Y
* Blocked by Dcm methylation.
BssHII  (512)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BstXI  (461)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (435)
1 site
A G A T C T T C T A G A
PsiI  (428)
1 site
T T A T A A A A T A T T
PvuI  (401)
1 site
C G A T C G G C T A G C
BsiWI  (366)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
BciVI  (356)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
PfoI  (332)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
TsoI  (272)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BspEI  (195)
1 site
T C C G G A A G G C C T
BsaI  (139)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
EciI  (51)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
NmeAIII  (47)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsiHKAI  (21)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (17)
1 site
G T G C A C C A C G T G
Start  (0)
0 sites
MBP
1 .. 1176  =  1176 bp
392 amino acids  =  42.9 kDa
   Segment 1:  signal sequence  
   1 .. 78  =  78 bp
   26 amino acids  =  2.7 kDa
Product: maltose binding protein from E. coli
This gene encodes a signal sequence that can direct
MBP to the bacterial periplasm.
MBP
1 .. 1176  =  1176 bp
392 amino acids  =  42.9 kDa
   Segment 2:  
   79 .. 1176  =  1098 bp
   366 amino acids  =  40.2 kDa
Product: maltose binding protein from E. coli
This gene encodes a signal sequence that can direct
MBP to the bacterial periplasm.
MBP
1 .. 1176  =  1176 bp
392 amino acids  =  42.9 kDa
2 segments
Product: maltose binding protein from E. coli
This gene encodes a signal sequence that can direct
MBP to the bacterial periplasm.
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