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Plasmid Files

SNAP-tag®

Human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives.

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SNAP-tag.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
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500 400 300 200 100 SNAP-tag® End (546) BssS α I (511) BanII (485) BmgBI (462) BaeGI - Bme1580I (419) BsrBI (404) Bpu10I (400) NaeI (363) BsrFI - NgoMIV (361) SfiI (358) SfcI (339) BseRI (244) Bsu36I (218) EciI (176) BtgI (128) BsaHI (125) EcoO109I (78) Start (0) SNAP-tag® 546 bp
End  (546)
0 sites
BssSαI  (511)
1 site
C A C G A G G T G C T C
BanII  (485)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BmgBI  (462)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BaeGI  (419)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (419)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsrBI  (404)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Bpu10I  (400)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NaeI  (363)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsrFI  (361)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NgoMIV  (361)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SfiI  (358)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SfcI  (339)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BseRI  (244)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Bsu36I  (218)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EciI  (176)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BtgI  (128)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsaHI  (125)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EcoO109I  (78)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
Start  (0)
0 sites
SNAP-tag®
1 .. 546  =  546 bp
182 amino acids  =  19.4 kDa
Product: human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives
fast-reacting variant, optimized for mammalian expression
SNAP-tag®
1 .. 546  =  546 bp
182 amino acids  =  19.4 kDa
Product: human O6-alkylguanine-DNA-alkyltransferase variant that forms covalent bonds with benzylguanine derivatives
fast-reacting variant, optimized for mammalian expression
ORF:  1 .. 546  =  546 bp
ORF:  182 amino acids  =  19.4 kDa
ORF:  1 .. 546  =  546 bp
ORF:  182 amino acids  =  19.4 kDa  (no start codon)
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