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Plasmid Files

SV40 promoter

Simian virus 40 (SV40) enhancer and early promoter, with the SV40 origin of replication.

 
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 300 200 100 SV40 promoter SV40 ori End (317) AvrII (302) StuI (301) BseRI (298) BglI - SfiI (255) BtgI - NcoI (209) BmrI (181) SexAI * (69) Start (0) SV40 promoter 317 bp
End  (317)
0 sites
AvrII  (302)
1 site
C C T A G G G G A T C C
StuI  (301)
1 site
A G G C C T T C C G G A
BseRI  (298)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BglI  (255)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (255)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BtgI  (209)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (209)
1 site
C C A T G G G G T A C C
BmrI  (181)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
SexAI  (69)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
Start  (0)
0 sites
SV40 promoter
1 .. 317  =  317 bp
SV40 enhancer and early promoter
SV40 promoter
1 .. 317  =  317 bp
SV40 enhancer and early promoter
SV40 ori
168 .. 303  =  136 bp
SV40 origin of replication
SV40 ori
168 .. 303  =  136 bp
SV40 origin of replication
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