lac operator

DNA sequence element that binds the lac repressor.
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15 10 5 End (17) BsrBI (7) Start (0) lac operator lac operator 17 bp
End  (17)
0 sites
BsrBI  (7)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Start  (0)
0 sites
lac operator
1 .. 17  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1 .. 17  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
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Download lac operator.dna file

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Individual Sequences & Maps

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