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Plasmid Files

pACYC184

Plasmid containing the p15A origin of replication and a tetracycline resistance gene.

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pACYC184 Sequence and MappACYC184.dna
Map and Sequence File   
Sequence Author:  New England Biolabs
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 BsrDI (4233) NcoI (3945) ScaI (3833) TatI (3831) BaeGI - Bme1580I (3783) DrdI - PflFI - Tth111I (3702) Bts α I (3619) Bsu36I (3593) BclI * (3542) BsaBI * (3446) AhdI (3209) BmeT110I (2921) AvaI - BsoBI (2920) BfuAI - BspMI (2549) BstAPI (2546) NruI (2469) PspFI (2448) BseYI (2444) EagI (2434) SalI (2146) EcoRI (1) BstZ17I (596) XmnI (639) PfoI * (788) SacII (835) BssS α I (960) AseI (1407) XbaI (1425) EarI (1453) BspDI - ClaI (1519) HindIII (1524) SfcI (1633) EcoRV (1682) BamHI (1870) BspHI (1984) SphI (2061) EcoNI (2121) pACYC184 4245 bp
BsrDI  (4233)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
NcoI  (3945)
1 site
C C A T G G G G T A C C
ScaI  (3833)
1 site
A G T A C T T C A T G A
TatI  (3831)
1 site
W G T A C W W C A T G W
BaeGI  (3783)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (3783)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
DrdI  (3702)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PflFI  (3702)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3702)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BtsαI  (3619)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
Bsu36I  (3593)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BclI  (3542)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsaBI  (3446)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
AhdI  (3209)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmeT110I  (2921)
1 site
C Y C G R G G R G C Y C
AvaI  (2920)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2920)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BfuAI  (2549)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2549)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BstAPI  (2546)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
NruI  (2469)
1 site
T C G C G A A G C G C T
PspFI  (2448)
1 site
C C C A G C G G G T C G
BseYI  (2444)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
EagI  (2434)
1 site
C G G C C G G C C G G C
SalI  (2146)
1 site
G T C G A C C A G C T G
EcoRI  (1)
1 site
G A A T T C C T T A A G
BstZ17I  (596)
1 site
G T A T A C C A T A T G
XmnI  (639)
1 site
G A A N N N N T T C C T T N N N N A A G
PfoI  (788)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SacII  (835)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BssSαI  (960)
1 site
C A C G A G G T G C T C
AseI  (1407)
1 site
A T T A A T T A A T T A
XbaI  (1425)
1 site
T C T A G A A G A T C T
EarI  (1453)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BspDI  (1519)
1 site
A T C G A T T A G C T A
ClaI  (1519)
1 site
A T C G A T T A G C T A
HindIII  (1524)
1 site
A A G C T T T T C G A A
SfcI  (1633)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
EcoRV  (1682)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BamHI  (1870)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BspHI  (1984)
1 site
T C A T G A A G T A C T
SphI  (2061)
1 site
G C A T G C C G T A C G
EcoNI  (2121)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
TcR
1581 .. 2771  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
1581 .. 2771  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
CmR
3805 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
3805 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
p15A ori
848 .. 1393  =  546 bp
Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
p15A ori
848 .. 1393  =  546 bp
Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
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