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pBC KS(+)

Phagemid vector derived from pBluescript II KS(+), with a chloramphenicol resistance gene. The MCS is reversed relative to pBC SK(+).

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pBC KS(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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XmnI (3084) BspEI (2636) SnaBI (2630) BsrDI (2619) BpmI (2516) MscI (2369) NcoI - StyI (2331) ScaI (2219) TatI (2217) BstBI (2103) PflFI - Tth111I (2087) AgeI (2027) Bsu36I (1978) BclI * (1913) PsiI (99) DraIII (227) NgoMIV (328) NaeI (330) BglI (472) FspI (479) PvuI (500) BmrI (580) Eco53kI (655) SacI (657) AleI (663) SacII (664) BstXI (665) EagI - NotI (670) XbaI (677) SpeI (683) BamHI (689) TspMI - XmaI (695) SmaI (697) PstI (705) EcoRI (707) EcoRV (715) HindIII (719) BspDI - ClaI (726) SalI (734) AccI (735) HincII (736) AbsI - PaeR7I - PspXI - XhoI (740) EcoO109I - PspOMI (749) ApaI (753) Acc65I (755) KpnI (759) BspQI - SapI (1037) AflIII - PciI (1153) NspI (1157) AlwNI (1569) pBC KS(+) 3400 bp
XmnI  (3084)
1 site
G A A N N N N T T C C T T N N N N A A G
BspEI  (2636)
1 site
T C C G G A A G G C C T
SnaBI  (2630)
1 site
T A C G T A A T G C A T
BsrDI  (2619)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BpmI  (2516)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
MscI  (2369)
1 site
T G G C C A A C C G G T
NcoI  (2331)
1 site
C C A T G G G G T A C C
StyI  (2331)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
ScaI  (2219)
1 site
A G T A C T T C A T G A
TatI  (2217)
1 site
W G T A C W W C A T G W
BstBI  (2103)
1 site
T T C G A A A A G C T T
PflFI  (2087)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2087)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AgeI  (2027)
1 site
A C C G G T T G G C C A
Bsu36I  (1978)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BclI  (1913)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PsiI  (99)
1 site
T T A T A A A A T A T T
DraIII  (227)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NgoMIV  (328)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (330)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BglI  (472)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (479)
1 site
T G C G C A A C G C G T
PvuI  (500)
1 site
C G A T C G G C T A G C
BmrI  (580)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Eco53kI  (655)
1 site
G A G C T C C T C G A G
SacI  (657)
1 site
G A G C T C C T C G A G
AleI  (663)
1 site
C A C N N N N G T G G T G N N N N C A C
SacII  (664)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI  (665)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EagI  (670)
1 site
C G G C C G G C C G G C
NotI  (670)
1 site
G C G G C C G C C G C C G G C G
XbaI  (677)
1 site
T C T A G A A G A T C T
SpeI  (683)
1 site
A C T A G T T G A T C A
BamHI  (689)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (695)
1 site
C C C G G G G G G C C C
XmaI  (695)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (697)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
PstI  (705)
1 site
C T G C A G G A C G T C
EcoRI  (707)
1 site
G A A T T C C T T A A G
EcoRV  (715)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HindIII  (719)
1 site
A A G C T T T T C G A A
BspDI  (726)
1 site
A T C G A T T A G C T A
ClaI  (726)
1 site
A T C G A T T A G C T A
SalI  (734)
1 site
G T C G A C C A G C T G
AccI  (735)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (736)
1 site
G T Y R A C C A R Y T G
AbsI  (740)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (740)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (740)
1 site
V C T C G A G B B G A G C T C V
XhoI  (740)
1 site
C T C G A G G A G C T C
EcoO109I  (749)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (749)
1 site
G G G C C C C C C G G G
ApaI  (753)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
Acc65I  (755)
1 site
G G T A C C C C A T G G
KpnI  (759)
1 site
G G T A C C C C A T G G
BspQI  (1037)
1 site
G C T C