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pBC SK(+)

Phagemid vector derived from pBluescript II SK(+), with a chloramphenicol resistance gene. The MCS is reversed relative to pBC KS(+).

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pBC SK(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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XmnI (3083) BclI * (3057) Bsu36I (2993) AgeI (2943) PflFI - Tth111I (2886) BstBI (2869) ScaI (2755) TatI (2753) NcoI - StyI (2639) MscI (2605) BsrDI (2357) SnaBI (2344) BspEI (2334) PsiI (99) DraIII (227) NgoMIV (328) NaeI (330) BglI (472) FspI (479) PvuI (500) BmrI (580) Acc65I (653) KpnI (657) PspOMI (659) EcoO109I (660) ApaI (663) AbsI - PaeR7I - PspXI - XhoI (668) SalI (674) AccI (675) HincII (676) BspDI - ClaI (684) HindIII (689) EcoRV (697) EcoRI (701) PstI (711) TspMI - XmaI (713) SmaI (715) BamHI (719) SpeI (725) XbaI (731) EagI - NotI (738) AleI (749) SacII (750) BstXI (751) Eco53kI (757) SacI (759) BspQI - SapI (1037) AflIII - PciI (1153) NspI (1157) BseYI (1457) PspFI (1461) AlwNI (1569) pBC SK(+) 3400 bp
XmnI  (3083)
1 site
G A A N N N N T T C C T T N N N N A A G
BclI  (3057)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Bsu36I  (2993)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AgeI  (2943)
1 site
A C C G G T T G G C C A
PflFI  (2886)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2886)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstBI  (2869)
1 site
T T C G A A A A G C T T
ScaI  (2755)
1 site
A G T A C T T C A T G A
TatI  (2753)
1 site
W G T A C W W C A T G W
NcoI  (2639)
1 site
C C A T G G G G T A C C
StyI  (2639)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (2605)
1 site
T G G C C A A C C G G T
BsrDI  (2357)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
SnaBI  (2344)
1 site
T A C G T A A T G C A T
BspEI  (2334)
1 site
T C C G G A A G G C C T
PsiI  (99)
1 site
T T A T A A A A T A T T
DraIII  (227)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NgoMIV  (328)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (330)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BglI  (472)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
FspI  (479)
1 site
T G C G C A A C G C G T
PvuI  (500)
1 site
C G A T C G G C T A G C
BmrI  (580)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Acc65I  (653)
1 site
G G T A C C C C A T G G
KpnI  (657)
1 site
G G T A C C C C A T G G
PspOMI  (659)
1 site
G G G C C C C C C G G G
EcoO109I  (660)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
ApaI  (663)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AbsI  (668)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (668)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (668)
1 site
V C T C G A G B B G A G C T C V
XhoI  (668)
1 site
C T C G A G G A G C T C
SalI  (674)
1 site
G T C G A C C A G C T G
AccI  (675)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (676)
1 site
G T Y R A C C A R Y T G
BspDI  (684)
1 site
A T C G A T T A G