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Plasmid Files

pDNR-LIB

Donor vector for transferring a cloned cDNA into an acceptor vector using Cre recombinase in the Creator™ system.

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pDNR-LIB.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BmtI (4114) NheI (4110) BglII (4082) MluI (4059) BssS α I (3831) BciVI (3806) PspFI (3704) BseYI (3700) ApaLI (3690) AlwNI (3595) BspHI (3284) AscI (3170) BfuAI - BspMI (3148) BstZ17I (2851) EarI (2486) StuI * (2481) MslI (2240) BsgI (2159) SalI (45) NdeI (61) TspMI - XmaI (66) SmaI (68) EcoRI (71) PstI - SbfI (93) MfeI (148) NmeAIII (285) PaeR7I - XhoI (311) HindIII (317) XbaI (323) BstXI (336) BmrI (344) PspOMI (346) ApaI (350) AvrII (384) SpeI (403) Eco53kI (517) SacI (519) ScaI (558) SspI (663) NcoI (670) PasI (740) PflMI * (746) BsmBI (747) BpmI (855) BsrDI (958) BspEI (975) ZraI (1262) AatII (1264) SacII (1645) BsaAI - SnaBI (1973) BsrGI (2038) pDNR-LIB 4161 bp
BmtI  (4114)
1 site
G C T A G C C G A T C G
NheI  (4110)
1 site
G C T A G C C G A T C G
BglII  (4082)
1 site
A G A T C T T C T A G A
MluI  (4059)
1 site
A C G C G T T G C G C A
BssSαI  (3831)
1 site
C A C G A G G T G C T C
BciVI  (3806)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
PspFI  (3704)
1 site
C C C A G C G G G T C G
BseYI  (3700)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
ApaLI  (3690)
1 site
G T G C A C C A C G T G
AlwNI  (3595)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BspHI  (3284)
1 site
T C A T G A A G T A C T
AscI  (3170)
1 site
G G C G C G C C C C G C G C G G
BfuAI  (3148)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3148)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BstZ17I  (2851)
1 site
G T A T A C C A T A T G
EarI  (2486)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
StuI  (2481)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
MslI  (2240)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsgI  (2159)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SalI  (45)
1 site</