pEX-1

PrecisionShuttle™ bacterial vector for inducible expression of an untagged protein.

Sequence Author: OriGene

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PsiI (4433) DraIII (4308) BsaAI (4305) XmnI (3755) ScaI (3636) TatI (3634) BsaI (3217) AhdI (3156) AlwNI (2679) EcoRI (186) lac operator RBS BsaBI * - BseRI - BspDI * - ClaI * (290) AscI (306) BglII (313) HindIII (320) NheI (331) BmtI (335) RsrII (337) MluI (343) NotI (355) AvaI - BsoBI - PaeR7I - PspXI - XhoI (361) PmeI (370) FseI (380) BlpI (410) StyI (432) EcoO109I (437) BtgI (488) SacII (491) PflMI (723) BstAPI (824) BclI * (1155) BstEII (1322) PspOMI (1348) ApaI (1352) HincII - HpaI (1647) BsmBI - Esp3I (1756) KasI (1780) NarI * (1781) SfoI (1782) PluTI (1784) BamHI (2134) pEX-1 4505 bp
PsiI  (4433)
1 site
T T A T A A A A T A T T
DraIII  (4308)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaAI  (4305)
1 site
Y A C G T R R T G C A Y
XmnI  (3755)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3636)
1 site
A G T A C T T C A T G A
TatI  (3634)
1 site
W G T A C W W C A T G W
BsaI  (3217)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3156)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2679)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
EcoRI  (186)
1 site
G A A T T C C T T A A G
BsaBI  (290)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BseRI  (290)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BspDI  (290)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (290)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
AscI  (306)
1 site
G G C G C G C C C C G C G C G G
BglII  (313)
1 site
A G A T C T T C T A G A
HindIII  (320)
1 site
A A G C T T T T C G A A
NheI  (331)
1 site
G C T A G C C G A T C G
BmtI  (335)
1 site
G C T A G C C G A T C G
RsrII  (337)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (343)
1 site
A C G C G T T G C G C A
NotI  (355)
1 site
G C G G C C G C C G C C G G C G
AvaI  (361)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (361)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (361)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (361)
1 site
V C T C G A G B B G A G C T C V
XhoI  (361)
1 site
C T C G A G G A G C T C
PmeI  (370)
1 site
G T T T A A A C C A A A T T T G
FseI  (380)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BlpI  (410)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
StyI  (432)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EcoO109I  (437)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BtgI  (488)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
SacII  (491)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PflMI  (723)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BstAPI  (824)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BclI  (1155)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (1322)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1348)
1 site
G G G C C C C C C G G G
ApaI  (1352)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
HincII  (1647)
1 site
G T Y R A C C A R Y T G
HpaI  (1647)
1 site
G T T A A C C A A T T G
BsmBI  (1756)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (1756)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
KasI  (1780)
1 site
G G C G C C C C G C G G
NarI  (1781)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1782)
1 site
G G C G C C C C G C G G
PluTI  (1784)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BamHI  (2134)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
lacI
791 .. 1873  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
791 .. 1873  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
3083 .. 3943  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3083 .. 3874  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3083 .. 3943  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3875 .. 3943  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3083 .. 3943  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2324 .. 2912  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2324 .. 2912  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4075 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4075 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
3944 .. 4048  =  105 bp
AmpR promoter
3944 .. 4048  =  105 bp
MCS
288 .. 382  =  95 bp
multiple cloning site
MCS
288 .. 382  =  95 bp
multiple cloning site
lacI promoter
713 .. 790  =  78 bp
lacI promoter
713 .. 790  =  78 bp
T7 terminator
421 .. 467  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
421 .. 467  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
237 .. 261  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
237 .. 261  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
218 .. 236  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
218 .. 236  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
281 .. 286  =  6 bp
ribosome binding site
RBS
281 .. 286  =  6 bp
ribosome binding site
ORF:  724 .. 1041  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1630 .. 1893  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  1996 .. 2223  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  914 .. 1873  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  3213 .. 3479  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1656 .. 1907  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  3083 .. 3943  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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