Resources
Plasmid Files

pEX1

Vector for bacterial expression of lacZ fusion proteins. For other reading frames, use pEX2 or pEX3.

 
To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

 BspEI * (124) EcoO109I (5758) ScaI (5262) BsaI (4843) AhdI (4782) BspQI - SapI (3773) BstAPI (3713) BsaAI (3643) PflFI - Tth111I (3636) XbaI (3256) PstI (3218) SalI (3208) BamHI (3202) SmaI (3199) TspMI - XmaI (3197) KasI (174) NarI * (175) SfoI (176) PluTI (178) Bsu36I (416) ApoI (688) Bpu10I (697) EcoRV (1305) DraIII (1382) BclI * (1538) BssHII - MauBI (1690) AfeI (2027) Eco53kI (2130) SacI (2132) BsiWI (2963) pEX1 5783 bp
BspEI  (124)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
EcoO109I  (5758)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
ScaI  (5262)
1 site
A G T A C T T C A T G A
BsaI  (4843)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4782)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (3773)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3773)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
BstAPI  (3713)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BsaAI  (3643)
1 site
Y A C G T R R T G C A Y
PflFI  (3636)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3636)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
XbaI  (3256)
1 site
T C T A G A A G A T C T
PstI  (3218)
1 site
C T G C A G G A C G T C
SalI  (3208)
1 site
G T C G A C C A G C T G
BamHI  (3202)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SmaI  (3199)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (3197)
1 site
C C C G G G G G G C C C
XmaI  (3197)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
KasI  (174)
1 site
G G C G C C C C G C G G
NarI  (175)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (176)
1 site
G G C G C C C C G C G G
PluTI  (178)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
Bsu36I  (416)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
ApoI  (688)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
Bpu10I  (697)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoRV  (1305)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
DraIII  (1382)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BclI  (1538)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BssHII  (1690)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (1690)
1 site
C G C G C G C G G C G C G C G C
AfeI  (2027)
1 site
A G C G C T T C G C G A
Eco53kI  (2130)
1 site
G A G C T C C T C G A G
SacI  (2132)
1 site
G A G C T C C T C G A G
BsiWI  (2963)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
cro-lacZ
85 .. 3240  =  3156 bp
1051 amino acids  =  119.1 kDa
   Segment 1:  cro  
   85 .. 108  =  24 bp
   8 amino acids  =  1.0 kDa
Product: Cro-β-galactosidase hybrid protein
cro-lacZ
85 .. 3240  =  3156 bp
1051 amino acids  =  119.1 kDa
   Segment 2:  
   109 .. 249  =  141 bp
   47 amino acids  =  5.2 kDa
Product: Cro-β-galactosidase hybrid protein
cro-lacZ
85 .. 3240  =  3156 bp
1051 amino acids  =  119.1 kDa
   Segment 3:  lacZ  
   250 .. 3195  =  2946 bp
   982 amino acids  =  111.5 kDa
Product: Cro-β-galactosidase hybrid protein
cro-lacZ
85 .. 3240  =  3156 bp
1051 amino acids  =  119.1 kDa
   Segment 4:  
   3196 .. 3240  =  45 bp
   14 amino acids  =  1.5 kDa
Product: Cro-β-galactosidase hybrid protein
cro-lacZ
85 .. 3240  =  3156 bp
1051 amino acids  =  119.1 kDa
4 segments
Product: Cro-β-galactosidase hybrid protein
AmpR
4709 .. 5569  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4709 .. 5500  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4709 .. 5569  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   5501 .. 5569  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4709 .. 5569  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
3950 .. 4538  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3950 .. 4538  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AmpR promoter
5570 .. 5674  =  105 bp
AmpR promoter
5570 .. 5674  =  105 bp
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter