Resources
Plasmid Files

pF25A ICE T7

Flexi® vector with an ampicillin resistance marker, for cell-free translation in an insect cell extract.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pF25A ICE T7 Sequence and MappF25A ICE T7.dna
Map and Sequence File   
Sequence Author:  Promega
Download Free Trial Get SnapGene Viewer

 T7 promoter BglII (3499) MluI (3380) BspEI (3167) DrdI (2511) PciI (2403) AgeI (2335) BstBI (2308) AhdI (2233) BpmI (2164) BglI (2115) AsiSI - PvuI - SgfI (90) BtgI - NcoI (93) PmeI (457) EcoRI (462) BanII (472) Acc65I (474) KpnI (478) BamHI (483) XbaI (489) SalI (495) AccI (496) HincII (497) PstI - SbfI (505) SphI (511) BlpI (570) AfeI (645) MfeI (676) PsiI (712) SwaI (777) EcoO109I (1034) NruI (1069) EagI - NotI (1074) StuI (1083) EarI (1434) XmnI (1633) FspI (2010) pF25A ICE T7 3947 bp
BglII  (3499)
1 site
A G A T C T T C T A G A
MluI  (3380)
1 site
A C G C G T T G C G C A
BspEI  (3167)
1 site
T C C G G A A G G C C T
DrdI  (2511)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PciI  (2403)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (2335)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BstBI  (2308)
1 site
T T C G A A A A G C T T
AhdI  (2233)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (2164)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BglI  (2115)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AsiSI  (90)
1 site
G C G A T C G C C G C T A G C G
PvuI  (90)
1 site
C G A T C G G C T A G C
SgfI  (90)
1 site
G C G A T C G C C G C T A G C G
BtgI  (93)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (93)
1 site
C C A T G G G G T A C C
PmeI  (457)
1 site
G T T T A A A C C A A A T T T G
EcoRI  (462)
1 site
G A A T T C C T T A A G
BanII  (472)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Acc65I  (474)
1 site
G G T A C C C C A T G G
KpnI  (478)
1 site
G G T A C C C C A T G G
BamHI  (483)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (489)
1 site
T C T A G A A G A T C T
SalI  (495)
1 site
G T C G A C C A G C T G
AccI  (496)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (497)
1 site
G T Y R A C C A R Y T G
PstI  (505)
1 site
C T G C A G G A C G T C
SbfI  (505)
1 site
C C T G C A G G G G A C G T C C
SphI  (511)
1 site
G C A T G C C G T A C G
BlpI  (570)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AfeI  (645)
1 site
A G C G C T T C G C G A
MfeI  (676)
1 site
C A A T T G G T T A A C
PsiI  (712)
1 site
T T A T A A A A T A T T
SwaI  (777)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
EcoO109I  (1034)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
NruI  (1069)
1 site
T C G C G A A G C G C T
EagI  (1074)
1 site
C G G C C G G C C G G C
NotI  (1074)
1 site
G C G G C C G C C G C C G G C G
StuI  (1083)
1 site
A G G C C T T C C G G A
EarI  (1434)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
XmnI  (1633)
1 site
G A A N N N N T T C C T T N N N N A A G
FspI  (2010)
1 site
T G C G C A A C G C G T
AmpR
1446 .. 2306  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1446 .. 1514  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1446 .. 2306  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1515 .. 2306  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1446 .. 2306  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
2464 .. 3052  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2464 .. 3052  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
polyhedrin 3' UTR
611 .. 983  =  373 bp
3' UTR from the Autographa californica
nucleopolyhedrovirus polyhedrin gene (Suzuki et al.,
2006)
polyhedrin 3' UTR
611 .. 983  =  373 bp
3' UTR from the Autographa californica
nucleopolyhedrovirus polyhedrin gene (Suzuki et al.,
2006)
barnase
117 .. 452  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus
amyloliquefaciens

The barnase gene is lethal in standard bacterial
transformation strains.
barnase
117 .. 452  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus
amyloliquefaciens

The barnase gene is lethal in standard bacterial
transformation strains.
cer region
3168 .. 3451  =  284 bp
ColE1-derived recombination site that helps to
maintain plasmids as monomers
cer region
3168 .. 3451  =  284 bp
ColE1-derived recombination site that helps to
maintain plasmids as monomers
AmpR promoter
1341 .. 1445  =  105 bp
AmpR promoter
1341 .. 1445  =  105 bp
rrnB T1 terminator
3681 .. 3767  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
3681 .. 3767  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
T7 terminator
1018 .. 1065  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
1018 .. 1065  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
polyhedrin 5' UTR
40 .. 85  =  46 bp
5' UTR from the Malacosoma neustria
nucleopolyhedrovirus polyhedrin gene (Ezure et al.,
2006)
polyhedrin 5' UTR
40 .. 85  =  46 bp
5' UTR from the Malacosoma neustria
nucleopolyhedrovirus polyhedrin gene (Ezure et al.,
2006)
poly(A)
988 .. 1017  =  30 bp
synthetic poly(A) tract
poly(A)
988 .. 1017  =  30 bp
synthetic poly(A) tract
rrnB T2 terminator
3859 .. 3886  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
rrnB T2 terminator
3859 .. 3886  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter