Resources
Plasmid Files

pFC7A (HQ)

Flexi® vector with an ampicillin resistance marker, for bacterial or in vitro expression of a protein with a C-terminal HQ metal affinity tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pFC7A (HQ) Sequence and MappFC7A (HQ).dna
Map and Sequence File   
Sequence Author:  Promega
Download Free Trial Get SnapGene Viewer

 T7 promoter BglII (2977) MluI (2858) BspEI (2645) DrdI (1989) PciI (1881) AgeI (1813) BstBI (1786) BanI (1758) AsiSI - PvuI - SgfI (65) BtgI - NcoI (68) AvaI - BsoBI - TspMI - XmaI (261) BmeT110I (262) SmaI (263) Eco53kI - EcoICRI (427) BanII - SacI (429) XbaI (451) SalI (457) AccI (458) HincII (459) PstI - SbfI (467) SphI (473) BlpI (532) EcoO109I (559) BsaAI - SnaBI (773) SspI (906) EarI (912) XmnI (1111) FspI (1488) BglI (1593) BpmI (1642) pFC7A (HQ) 3425 bp
BglII  (2977)
1 site
A G A T C T T C T A G A
MluI  (2858)
1 site
A C G C G T T G C G C A
BspEI  (2645)
1 site
T C C G G A A G G C C T
DrdI  (1989)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PciI  (1881)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (1813)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BstBI  (1786)
1 site
T T C G A A A A G C T T
BanI  (1758)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
AsiSI  (65)
1 site
G C G A T C G C C G C T A G C G
PvuI  (65)
1 site
C G A T C G G C T A G C
SgfI  (65)
1 site
G C G A T C G C C G C T A G C G
BtgI  (68)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (68)
1 site
C C A T G G G G T A C C
AvaI  (261)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (261)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
TspMI  (261)
1 site
C C C G G G G G G C C C
XmaI  (261)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BmeT110I  (262)
1 site
C Y C G R G G R G C Y C
SmaI  (263)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Eco53kI  (427)
1 site
G A G C T C C T C G A G
EcoICRI  (427)
1 site
G A G C T C C T C G A G
BanII  (429)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (429)
1 site
G A G C T C C T C G A G
XbaI  (451)
1 site
T C T A G A A G A T C T
SalI  (457)
1 site
G T C G A C C A G C T G
AccI  (458)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (459)
1 site
G T Y R A C C A R Y T G
PstI  (467)
1 site
C T G C A G G A C G T C
SbfI  (467)
1 site
C C T G C A G G G G A C G T C C
SphI  (473)
1 site
G C A T G C C G T A C G
BlpI  (532)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (559)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaAI  (773)
1 site
Y A C G T R R T G C A Y
SnaBI  (773)
1 site
T A C G T A A T G C A T
SspI  (906)
1 site
A A T A T T T T A T A A
EarI  (912)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
XmnI  (1111)
1 site
G A A N N N N T T C C T T N N N N A A G
FspI  (1488)
1 site
T G C G C A A C G C G T
BglI  (1593)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BpmI  (1642)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AmpR
924 .. 1784  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   924 .. 992  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
924 .. 1784  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   993 .. 1784  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
924 .. 1784  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
1942 .. 2530  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1942 .. 2530  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
barnase
70 .. 405  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus
amyloliquefaciens

The barnase gene is lethal in standard bacterial
transformation strains.
barnase
70 .. 405  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus
amyloliquefaciens

The barnase gene is lethal in standard bacterial
transformation strains.
cer region
2646 .. 2929  =  284 bp
ColE1-derived recombination site that helps to
maintain plasmids as monomers
cer region
2646 .. 2929  =  284 bp
ColE1-derived recombination site that helps to
maintain plasmids as monomers
AmpR promoter
819 .. 923  =  105 bp
AmpR promoter
819 .. 923  =  105 bp
rrnB T1 terminator
3159 .. 3245  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
3159 .. 3245  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
T7 terminator
543 .. 590  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
543 .. 590  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
rrnB T2 terminator
3337 .. 3364  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
rrnB T2 terminator
3337 .. 3364  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
HQ tag
430 .. 447  =  18 bp
6 amino acids  =  813.9 Da
Product: HQHQHQ metal affinity tag
The HQ tag binds to nickel and other metal ions
much like the 6xHis tag, but may allow elution at
lower concentrations of imidazole (Godat et al.,
2008).
HQ tag
430 .. 447  =  18 bp
6 amino acids  =  813.9 Da
Product: HQHQHQ metal affinity tag
The HQ tag binds to nickel and other metal ions
much like the 6xHis tag, but may allow elution at
lower concentrations of imidazole (Godat et al.,
2008).
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter