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Plasmid Files

pHP45Ω-Tc

Plasmid carrying an Ω fragment for in vitro insertional mutagenesis (interposon) with a tetracycline resistance gene.

 
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pHP45(omega)-Tc.dna
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AatII (4427) ZraI (4425) SspI (4309) XmnI (4104) ScaI (3985) PvuI (3875) PstI (3750) BsaI (3566) AhdI (3505) AlwNI (3028) AflIII - PciI (2612) BspQI - SapI (2496) NdeI (2435) BstZ17I (2385) PflFI - Tth111I (2359) PfoI (2256) BsmBI (2255) EcoRI (1) SmaI (8) BamHI (11) HindIII (32) DraIII (446) EcoRV (634) NheI (676) BmtI (680) FspAI (709) BamHI (822) SgrAI (857) BtgI (975) SphI (1013) EcoNI (1073) SalI (1098) PshAI (1163) EagI (1386) NruI (1421) BfuAI - BspMI (1501) BsmI (1806) BsaBI * (1920) HindIII (2140) BamHI (2161) SmaI (2168) EcoRI (2171) BspDI - ClaI (2197) HindIII (2202) pHP45 Ω- Tc 4497 bp
AatII  (4427)
1 site
G A C G T C C T G C A G
ZraI  (4425)
1 site
G A C G T C C T G C A G
SspI  (4309)
1 site
A A T A T T T T A T A A
XmnI  (4104)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3985)
1 site
A G T A C T T C A T G A
PvuI  (3875)
1 site
C G A T C G G C T A G C
PstI  (3750)
1 site
C T G C A G G A C G T C
BsaI  (3566)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3505)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3028)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2612)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2612)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2496)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2496)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NdeI  (2435)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2385)
1 site
G T A T A C C A T A T G
PflFI  (2359)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2359)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2256)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BsmBI  (2255)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
EcoRI  (1)
2 sites
G A A T T C C T T A A G
SmaI  (8)
2 sites
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (11)
3 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
HindIII  (32)
3 sites
A A G C T T T T C G A A
DraIII  (446)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
EcoRV  (634)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NheI  (676)
1 site
G C T A G C C G A T