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Plasmid Files

pHP45Ω

Plasmid carrying an Ω fragment for in vitro insertional mutagenesis (interposon) with a spectinomycin/streptomycin resistance gene.

 
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pHP45(omega).dna
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AatII (4333) ZraI (4331) SspI (4215) HincII (3952) ScaI (3891) PstI (3656) FspI (3633) AhdI (3411) AflIII - PciI (2518) BspQI - SapI (2402) BstAPI (2342) NdeI (2341) BstZ17I (2291) AccI (2290) PflFI - Tth111I (2265) EcoRI (1) SmaI (8) BamHI (11) HindIII (32) BssHII (472) NgoMIV (547) NaeI (549) BtgZI (638) BfuAI - BspMI (849) BlpI (888) BclI * (998) BstEII (1035) EagI (1669) XcmI (1765) SphI (1782) BsaBI * (1826) BanII (1864) HindIII (2046) BamHI (2067) SmaI (2074) EcoRI (2077) BspDI - ClaI (2103) HindIII (2108) BsmBI (2161) PfoI (2162) pHP45 Ω 4403 bp
AatII  (4333)
1 site
G A C G T C C T G C A G
ZraI  (4331)
1 site
G A C G T C C T G C A G
SspI  (4215)
1 site
A A T A T T T T A T A A
HincII  (3952)
1 site
G T Y R A C C A R Y T G
ScaI  (3891)
1 site
A G T A C T T C A T G A
PstI  (3656)
1 site
C T G C A G G A C G T C
FspI  (3633)
1 site
T G C G C A A C G C G T
AhdI  (3411)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AflIII  (2518)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2518)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2402)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2402)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2342)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
NdeI  (2341)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstZ17I  (2291)
1 site
G T A T A C C A T A T G
AccI  (2290)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PflFI  (2265)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2265)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
EcoRI  (1)
2 sites
G A A T T C C T T A A G
SmaI  (8)
2 sites
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (11)
2 sites
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
HindIII  (32)
3 sites
A A G C T T T T C G A A
BssHII  (472)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NgoMIV  (547)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (549)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BtgZI  (638)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BfuAI  (849)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (849)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (888)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BclI  (998)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (1035)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EagI  (1669)
1 site
C G G C C G G C C G G C
XcmI  (1765)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SphI  (1782)
1 site
G C A T G C C G T A C G
BsaBI  (1826)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BanII  (1864)
1 site
G R G C Y C C Y C G R G