Resources
Plasmid Files

pMAL-c5E

Bacterial vector for inducible cytoplasmic expression of maltose-binding protein (MBP) fusions with an enterokinase cleavage site.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pMAL-c5E.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
Download Free Trial Get SnapGene Viewer


FspAI (5645) Bpu10I (5517) PflFI - Tth111I (4880) BspQI - SapI (4799) PciI (4682) Bsu36I (3993) BglI (3759) ScaI (3396) TatI (3394) DraI (3299) BspHI (3037) RsrII (2993) HindIII (2760) MscI * (5655) PflMI (13) MluI (431) BstEII (612) PspOMI (638) ApaI (642) HpaI (937) KasI (1070) NarI * (1071) SfoI (1072) PluTI (1074) MfeI (1502) BsiWI (1818) PsiI (1880) BglII (1887) BmgBI (2070) BsmI (2148) BlpI (2314) Eco53kI (2636) SacI (2638) AvaI - BsoBI (2671) BmeT110I (2672) Acc65I (2691) KpnI (2695) NdeI (2699) NcoI - StyI (2705) EagI - NotI (2712) EcoRV (2721) SalI - SgrDI (2725) AccI (2726) BamHI (2731) EcoRI (2737) PstI - SbfI (2748) pMAL-c5E 5680 bp
FspAI  (5645)
1 site
R T G C G C A Y Y A C G C G T R
Bpu10I  (5517)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflFI  (4880)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4880)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BspQI  (4799)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4799)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (4682)
1 site
A C A T G T T G T A