Resources
Plasmid Files

pMAL-c5X-His

Bacterial vector for inducible cytoplasmic expression of maltose-binding protein (MBP) fusions with a Factor Xa cleavage site and a C-terminal 6xHis tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pMAL-c5X-His.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
Download Free Trial Get SnapGene Viewer


MscI * (5670) FspAI (5660) Bpu10I (5532) PflFI - Tth111I (4895) BspQI - SapI (4814) PciI (4697) Bsu36I (4008) BglI (3774) ScaI (3411) TatI (3409) DraI (3314) BspHI (3052) RsrII (3008) PflMI (13) MluI (431) BstEII (612) PspOMI (638) ApaI (642) HpaI (937) KasI (1070) NarI * (1071) SfoI (1072) PluTI (1074) MfeI (1502) BsiWI (1818) PsiI (1880) BglII (1887) BmgBI (2070) BsmI (2148) BlpI (2314) Eco53kI (2636) SacI (2638) AvaI - BsoBI (2671) BmeT110I (2672) XmnI (2688) NdeI (2696) NcoI - StyI (2702) EagI - NotI (2709) EcoRV (2718) SalI - SgrDI (2722) AccI (2723) BamHI (2728) EcoRI (2734) PstI - SbfI (2745) HindIII (2775) pMAL-c5X-His 5695 bp
MscI  (5670)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
FspAI  (5660)
1 site
R T G C G C A Y Y A C G C G T R
Bpu10I  (5532)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflFI  (4895)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4895)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BspQI  (4814)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4814)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (4697)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
Bsu36I  (4008)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BglI  (3774)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
ScaI  (3411)
1 site
A G T A C T T C<