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pMAL-p5G

Bacterial vector for inducible periplasmic expression of maltose-binding protein (MBP) fusions with a Genenase™ I cleavage site.

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pMAL-p5G.dna
Map and Sequence File:    Download    Open   
Sequence Author:  New England Biolabs
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MscI * (5730) FspAI (5720) Bpu10I (5592) PflFI - Tth111I (4955) BspQI - SapI (4874) PciI (4757) Bsu36I (4068) BglI (3834) ScaI (3471) TatI (3469) DraI (3374) BspHI (3112) RsrII (3068) HindIII (2835) PflMI (13) MluI (431) BstEII (612) PspOMI (638) ApaI (642) HpaI (937) KasI (1070) NarI * (1071) SfoI (1072) PluTI (1074) MfeI (1502) BsiWI (1893) PsiI (1955) BglII (1962) BmgBI (2145) BsmI (2223) BlpI (2389) Eco53kI (2711) SacI (2713) AvaI - BsoBI (2746) BmeT110I (2747) BsaAI - SnaBI (2769) NdeI (2774) NcoI - StyI (2780) EagI - NotI (2787) EcoRV (2796) SalI - SgrDI (2800) AccI (2801) BamHI (2806) EcoRI (2812) PstI - SbfI (2823) pMAL-p5G 5755 bp
MscI  (5730)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
FspAI  (5720)
1 site
R T G C G C A Y Y A C G C G T R
Bpu10I  (5592)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflFI  (4955)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4955)
1 site
G A C N N N G T