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Plasmid Files

pMCS5

E. coli cloning vector with an extensive multiple cloning site containing 59 unique restriction sites.

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pMCS5.dna
Map and Sequence File:    Download    Open   
Sequence Author:  MoBiTec
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SphI (205) BfuAI - BspMI (200) HindIII (195) RsrII (189) AvrII (182) I-SceI (180) PsiI (2973) DraIII (2848) BtgZI (2840) XmnI (2295) ScaI (2176) TatI (2174) NmeAIII (1844) BpmI (1766) BsaI (1757) AhdI (1696) PstI - SbfI (211) SalI (213) AccI (214) XbaI (219) BamHI (225) PmlI (233) TspMI - XmaI (237) SmaI - SrfI (239) PmeI (247) MfeI (252) BstBI (259) BsiWI (264) NruI (272) PaeR7I - PspXI - XhoI (276) SnaBI (284) Acc65I (288) KpnI (292) Eco53kI (296) SacI (298) EcoRI (300) EagI - NotI (307) SacII (313) PspOMI (314) ApaI (318) NdeI (320) SfiI (322) AgeI - SgrAI (329) FseI (341) BglII (344) NheI (350) BmtI (354) SpeI (356) KasI (362) NarI (363) SfoI (364) PluTI (366) MluI (368) EcoRV (376) NsiI (384) HpaI (388) BspDI * - ClaI * (393) NcoI (398) AscI - BssHII (404) PacI (415) SwaI (422) BspQI - SapI (687) PciI (803) BseYI (1107) PspFI (1111) AlwNI (1219) pMCS5 3081 bp
SphI  (205)
1 site
G C A T G C C G T A C G
BfuAI  (200)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (200)
1 site
A C C T G C ( N ) 4 T G