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Plasmid Files

pMMB207

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a chloramphenicol resistance gene. See also pMMB208.

 
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pMMB207.dna
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HindIII (9046) BbuI - PaeI - SphI (9044) PstI - SbfI - SdaI - Sse8387I (9038) BfmI - BstSFI - SfcI (9034) SalI (9028) XbaI (9022) BamHI (9016) SmaI (9013) Cfr9I - KpnI - TspMI - XmaI - XmaCI (9011) Acc65I - Asp718I (9007) Psp124BI - SacI - SstI (9005) Ecl136II - Eco53kI - EcoICRI (9003) EcoRI (8995) MauBI (8720) MluI (8263) ApaI (8060) Bsp120I - PspOMI (8056) BstHPI - HpaI - KspAI (7761) BspQI - LguI - PciSI - SapI (7455) FspAI (6984) BstENI - EcoNI - XagI (6834) PfoI * (6694) SgrAI (4746) Asp700I - MroXI - PdmI - XmnI (0) BsaI - Bso31I - BspTNI - Eco31I (148) BauI - BssS α I - Bst2BI (612) AccIII - Aor13HI - BseAI - Bsp13I - BspEI - Kpn2I - MroI (1256) BstSNI - Eco105I - SnaBI (1266) SfiI (1886) DraII - EcoO109I (2438) BssNAI - Bst1107I - BstZ17I (3261) pMMB207 9076 bp
HindIII  (9046)
1 site
A A G C T T T T C G A A
BbuI  (9044)
1 site
G C A T G C C G T A C G
PaeI  (9044)
1 site
G C A T G C C G T A C G
SphI  (9044)
1 site
G C A T G C C G T A C G
PstI  (9038)
1 site
C T G C A G G A C G T C
SbfI  (9038)
1 site
C C T G C A G G G G A C G T C C
SdaI  (9038)
1 site
C C T G C A G G G G A C G T C C
Sse8387I  (9038)
1 site
C C T G C A G G G G A C G T C C
BfmI  (9034)
1 site
C T R Y A G G A Y R T C

Sticky ends from different BfmI sites may not be compatible.
BstSFI  (9034)
1 site
C T R Y A G G A Y R T C

Sticky ends from different BstSFI sites may not be compatible.
SfcI  (9034)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SalI  (9028)
1 site
G T C G A C C A G C T G
XbaI  (9022)
1 site
T C T A G A A G A T C T
BamHI  (9016)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (9013)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Cfr9I  (9011)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the Cfr9I recognition sequence.
KpnI  (9011)
1 site
G G T A C C C C A T G G
TspMI  (9011)
1 site
C C C G G G G G G C C C
XmaI  (9011)
1 site