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pMMB67EH

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and an ampicillin resistance gene. See also pMMB67HE.

 
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pMMB67EH.dna
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BbuI - PaeI - SphI (8827) PstI - SbfI - SdaI - Sse8387I (8821) SalI (8811) XbaI (8805) BamHI (8799) SmaI (8796) Cfr9I - KpnI - TspMI - XmaI - XmaCI (8794) Acc65I - Asp718I (8790) Psp124BI - SacI - SstI (8788) Ecl136II - Eco53kI - EcoICRI (8786) EcoRI (8778) MauBI (8503) MluI (8046) ApaI (7843) Bsp120I - PspOMI (7839) BstHPI - HpaI - KspAI (7544) BsmBI - Esp3I (7431) BspQI - LguI - PciSI - SapI (7238) FspAI (6767) BsaAI - BstBAI - Ppu21I (6712) BstENI - EcoNI - XagI (6617) PfoI * (6477) SgrAI (4529) HindIII (0) BauI - BssS α I - Bst2BI (642) DraI (733) BpvUI - Ple19I - PvuI (942) AhdI - AspEI - BmeRI - DriI - Eam1105I (1311) CsiI - MabI - SexAI * (1435) SfiI (1669) DraII - EcoO109I (2221) BssNAI - Bst1107I - BstZ17I (3044) PasI (4050) pMMB67EH 8829 bp
BbuI  (8827)
1 site
G C A T G C C G T A C G
PaeI  (8827)
1 site
G C A T G C C G T A C G
SphI  (8827)
1 site
G C A T G C C G T A C G
PstI  (8821)
1 site
C T G C A G G A C G T C
SbfI  (8821)
1 site
C C T G C A G G G G A C G T C C
SdaI  (8821)
1 site
C C T G C A G G G G A C G T C C
Sse8387I  (8821)
1 site
C C T G C A G G G G A C G T C C
SalI  (8811)
1 site
G T C G A C C A G C T G
XbaI  (8805)
1 site
T C T A G A A G A T C T
BamHI  (8799)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8796)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Cfr9I  (8794)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the Cfr9I recognition sequence.
KpnI  (8794)
1 site
G G T A C C C C A T G G
TspMI  (8794)
1 site
C C C G G G G G G C C C
XmaI  (8794)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
XmaCI  (8794)
1 site
C C C G G G G G G C C C
Acc65I  (8790)
1 site
G G T A C C C C A T G G
Asp718I  (8790)
1 site
G G T A C C C C A T G G
Psp124BI  (8788)
1 site
G A G C T C C T C G A G
SacI  (8788)
1 site
G A G C T C C T C G A G
SstI  (8788)
1 site
G A G C T C C T C G A G
Ecl136II  (8786)
1 site
G A G C T C C T C G A G
Eco53kI  (8786)
1 site
G A G C T C C T C G A G
EcoICRI  (8786)
1 site
G A G C T C C T C G A G
EcoRI  (8778)
1 site
G A A T T C C T T A A G
MauBI  (8503)
1 site
C G C G C G C G G C G C G C G C
MluI  (8046)
1 site
A C G C G T T G C G C A
ApaI  (7843)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
Bsp120I  (7839)
1 site
G G G C C C C C C G G G
PspOMI  (7839)
1 site
G G G C C C C C C G G G
BstHPI  (7544)
1 site
G T T A A C C A A T T G
HpaI  (7544)
1 site
G T T A A C C A A T T G
KspAI  (7544)
1 site
G T T A A C C A A T T G
BsmBI  (7431)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
Esp3I  (7431)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
For full activity, add fresh DTT.
BspQI  (7238)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
LguI  (7238)
1 site