pOSIP-CH

Prokaryotic one-step cloning and chromosomal integration vector encoding a chloramphenicol resistance marker and the HK022 integrase.
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
Acc65I (13) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (5569) BseRI (5221) BmtI (5097) NheI (5093) NdeI (4824) SalI (4326) ScaI (4175) SspI (4070) PasI (3990) BpmI (3880) BspEI (3754) PvuII (3658) DraIII (3415) KpnI (17) BamHI (22) BssHII (70) BsrGI (198) SrfI (225) BsaI (363) BglII (469) BciVI (923) SpeI (1126) PstI (1144) SphI (1150) PaeR7I - XhoI (1152) KasI (1164) NarI (1165) SfoI (1166) PluTI (1168) BstBI (1234) MfeI (1239) PacI (1340) AgeI (1343) PspOMI (1349) ApaI (1353) ZraI (1379) AatII (1381) AvrII (1418) BstAPI (1965) PshAI (2090) AflIII - MluI (2240) KflI - PpuMI (2350) TaqII (2526) NruI (2828) BtsI - BtsαI (3076) pOSIP-CH 6398 bp
Acc65I  (13)
1 site
G G T A C C C C A T G G
PflFI  (5569)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5569)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BseRI  (5221)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BmtI  (5097)
1 site
G C T A G C C G A T C G
NheI  (5093)
1 site
G C T A G C C G A T C G
NdeI  (4824)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SalI  (4326)
1 site
G T C G A C C A G C T G
ScaI  (4175)
1 site
A G T A C T T C A T G A
SspI  (4070)
1 site
A A T A T T T T A T A A
PasI  (3990)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BpmI  (3880)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BspEI  (3754)
1 site
T C C G G A A G G C C T
PvuII  (3658)
1 site
C A G C T G G T C G A C
DraIII  (3415)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
KpnI  (17)
1 site
G G T A C C C C A T G G
BamHI  (22)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BssHII  (70)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BsrGI  (198)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
SrfI  (225)
1 site
G C C C G G G C C G G G C C C G
BsaI  (363)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglII  (469)
1 site
A G A T C T T C T A G A
BciVI  (923)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
SpeI  (1126)
1 site
A C T A G T T G A T C A
PstI  (1144)
1 site
C T G C A G G A C G T C
SphI  (1150)
1 site
G C A T G C C G T A C G
PaeR7I  (1152)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1152)
1 site
C T C G A G G A G C T C
KasI  (1164)
1 site
G G C G C C C C G C G G
NarI  (1165)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1166)
1 site
G G C G C C C C G C G G
PluTI  (1168)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BstBI  (1234)
1 site
T T C G A A A A G C T T
MfeI  (1239)
1 site
C A A T T G G T T A A C
PacI  (1340)
1 site
T T A A T T A A A A T T A A T T
AgeI  (1343)
1 site
A C C G G T T G G C C A
PspOMI  (1349)
1 site
G G G C C C C C C G G G
ApaI  (1353)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
ZraI  (1379)
1 site
G A C G T C C T G C A G
AatII  (1381)
1 site
G A C G T C C T G C A G
AvrII  (1418)
1 site
C C T A G G G G A T C C
BstAPI  (1965)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PshAI  (2090)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AflIII  (2240)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (2240)
1 site
A C G C G T T G C G C A
KflI  (2350)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (2350)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
TaqII  (2526)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
NruI  (2828)
1 site
T C G C G A A G C G C T
BtsI  (3076)
1 site
G C A G T G N N C G T C A C
BtsαI  (3076)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
HK022 integrase
2270 .. 3343  =  1074 bp
357 amino acids  =  40.4 kDa
Product: integrase from phage HK022
HK022 integrase
2270 .. 3343  =  1074 bp
357 amino acids  =  40.4 kDa
Product: integrase from phage HK022
λ repressor (ts)
1424 .. 2137  =  714 bp
237 amino acids  =  26.2 kDa
Product: temperature-sensitive variant of the phage λ repressor
thermosensitivity is conferred by the A67T mutation
λ repressor (ts)
1424 .. 2137  =  714 bp
237 amino acids  =  26.2 kDa
Product: temperature-sensitive variant of the phage λ repressor
thermosensitivity is conferred by the A67T mutation
CmR
3545 .. 4204  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
3545 .. 4204  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
477 .. 1065  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
477 .. 1065  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
R6K γ ori
4376 .. 4764  =  389 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
R6K γ ori
4376 .. 4764  =  389 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
ccdB
142 .. 447  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
142 .. 447  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
λ tL3 terminator
5113 .. 5359  =  247 bp
transcription terminator tL3 from phage λ
λ tL3 terminator
5113 .. 5359  =  247 bp
transcription terminator tL3 from phage λ
HK022 attP
4852 .. 5078  =  227 bp
attachment site of phage HK022
HK022 attP
4852 .. 5078  =  227 bp
attachment site of phage HK022
cat promoter
3442 .. 3544  =  103 bp
promoter of the E. coli cat gene
cat promoter
3442 .. 3544  =  103 bp
promoter of the E. coli cat gene
lambda t0 terminator
4225 .. 4319  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
4225 .. 4319  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
5679 .. 5765  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
5679 .. 5765  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
5860 .. 5946  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
5860 .. 5946  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6041 .. 6127  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6041 .. 6127  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6222 .. 6308  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6222 .. 6308  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
MCS 1
13 .. 75  =  63 bp
multiple cloning site, part 1
MCS 1
13 .. 75  =  63 bp
multiple cloning site, part 1
FRT
4775 .. 4822  =  48 bp
FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011).
FRT
4775 .. 4822  =  48 bp
FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011).
MCS 2
1126 .. 1169  =  44 bp
multiple cloning site, part 2
MCS 2
1126 .. 1169  =  44 bp
multiple cloning site, part 2
FRT (minimal)
1384 .. 1417  =  34 bp
supports FLP-mediated excision but not integration (Turan and Bode, 2011)
FRT (minimal)
1384 .. 1417  =  34 bp
supports FLP-mediated excision but not integration (Turan and Bode, 2011)
tonB terminator
1276 .. 1307  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
1276 .. 1307  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
rrnB T2 terminator
1200 .. 1227  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
1200 .. 1227  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
ORF:  142 .. 447  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  2270 .. 3343  =  1074 bp
ORF:  357 amino acids  =  40.4 kDa
ORF:  3545 .. 4204  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  3150 .. 3407  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  4122 .. 4373  =  252 bp
ORF:  83 amino acids  =  9.0 kDa
ORF:  5595 .. 5834  =  240 bp
ORF:  79 amino acids  =  9.2 kDa
ORF:  1424 .. 2137  =  714 bp
ORF:  237 amino acids  =  26.2 kDa
ORF:  2659 .. 2940  =  282 bp
ORF:  93 amino acids  =  9.8 kDa
Click here to try SnapGene

Download pOSIP-CH.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.