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pPCR-Script Cam SK(+)

Bacterial cloning vector with a chloramphenicol resistance marker and an SrfI site for inserting blunt PCR products.

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pPCR-Script Cam SK(+).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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XmnI (3083) BclI * (3057) Bsu36I (2993) AgeI (2943) PflFI - Tth111I (2886) BstBI (2869) ScaI (2755) TatI (2753) NcoI - StyI (2639) MscI (2605) BsrDI (2357) SnaBI (2344) BspEI (2334) PsiI (99) DraIII (227) NgoMIV (328) NaeI (330) BglI (472) FspI (479) PvuI (500) BmrI (580) Acc65I (653) KpnI (657) PspOMI (659) EcoO109I (660) ApaI (663) AbsI - PaeR7I - PspXI - XhoI (668) SalI (674) AccI (675) HincII (676) BspDI - ClaI (684) HindIII (689) EcoRV (697) EcoRI (701) PstI (711) BamHI (719) SrfI (728) EagI - NotI (738) AleI (749) SacII (750) BstXI (751) Eco53kI (757) SacI (759) BspQI - SapI (1037) AflIII - PciI (1153) NspI (1157) BseYI (1457) PspFI (1461) AlwNI (1569) pPCR-Script Cam SK(+) 3400 bp
XmnI  (3083)
1 site
G A A N N N N T T C C T T N N N N A A G
BclI  (3057)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Bsu36I  (2993)
1 site