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Plasmid Files

pRACE (linearized)

Linearized vector for In-Fusion® cloning of products amplified with the SMARTer RACE 5'/3' kit.

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pRACE (linearized) Sequence and MappRACE (linearized).dna
Map and Sequence File   
Sequence Author:  Clontech
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 End (2656) EcoRI (2636) Start (0) HindIII (1) In-Fusion homology to be added to GSP pRACE 2656 bp
End  (2656)
0 sites
EcoRI  (2636)
1 site
G A A T T C C T T A A G
Start  (0)
0 sites
HindIII  (1)
1 site
A A G C T T T T C G A A
AmpR
1180 .. 2040  =  861 bp
beta-lactamase, confers resistance to ampicillin,
carbenicillin, and related antibiotics
AmpR
1180 .. 2040  =  861 bp
beta-lactamase, confers resistance to ampicillin,
carbenicillin, and related antibiotics
ori
421 .. 1009  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
421 .. 1009  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AmpR promoter
2041 .. 2145  =  105 bp
AmpR promoter
AmpR promoter
2041 .. 2145  =  105 bp
AmpR promoter
lac promoter
67 .. 97  =  31 bp
lac promoter
67 .. 97  =  31 bp
M13 rev
19 .. 35  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
19 .. 35  =  17 bp
common sequencing primer, one of multiple similar
variants
lac operator
43 .. 59  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG).
lac operator
43 .. 59  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG).
M13 fwd
2619 .. 2635  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
2619 .. 2635  =  17 bp
common sequencing primer, one of multiple similar
variants
In-Fusion homology to be added to GSP
1 .. 15  =  15 bp
In-Fusion homology to be added to Gene-Specific
Primer (GSP)                            
In-Fusion homology to be added to GSP
1 .. 15  =  15 bp
In-Fusion homology to be added to Gene-Specific
Primer (GSP)                            
In-Fusion homology to UPM
2642 .. 2656  =  15 bp
In-Fusion homology to Universal Primer Mix (UPM)
                        
In-Fusion homology to UPM
2642 .. 2656  =  15 bp
In-Fusion homology to Universal Primer Mix (UPM)
                        
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