Resources
Plasmid Files

pRSET A

Bacterial vector for high-level expression of proteins with a cleavable 6xHis tag. For other reading frames, use pRSET B or pRSET C.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pRSET A Sequence and MappRSET A.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 NdeI (98) XbaI (58) BspQI - SapI (2834) NspI (2721) AflIII - PciI (2717) PspFI (2417) BseYI (2413) AlwNI (2308) AhdI (1829) BmrI (1789) BpmI (1760) NmeAIII (1682) ATG 6xHis NheI (136) BmtI (140) T7 tag (gene 10 leader) Xpress™ tag BspDI * - ClaI * (195) BamHI (202) Eco53kI (210) AvaI - BsoBI - PaeR7I - XhoI (211) BmeT110I - SacI (212) BglII (215) PstI (223) PvuII (224) MCS Acc65I (227) BtgI - KpnI - NcoI (231) EcoRI (236) BstBI (240) HindIII (243) BlpI (301) EcoO109I (328) BspEI * (378) NgoMIV (581) NaeI (583) BtgZI (681) BsaAI (686) DraIII (689) PsiI (814) XmnI (1229) BsaHI (1289) TatI (1346) ScaI (1348) pRSET A 2897 bp
NdeI  (98)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
XbaI  (58)
1 site
T C T A G A A G A T C T
BspQI  (2834)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2834)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
NspI  (2721)
1 site
R C A T G Y Y G T A C R
AflIII  (2717)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2717)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (2417)
1 site
C C C A G C G G G T C G
BseYI  (2413)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
AlwNI  (2308)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1829)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmrI  (1789)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
BpmI  (1760)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (1682)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NheI  (136)
1 site
G C T A G C C G A T C G
BmtI  (140)
1 site
G C T A G C C G A T C G
BspDI  (195)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (195)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BamHI  (202)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
Eco53kI  (210)
1 site
G A G C T C C T C G A G
AvaI  (211)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (211)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
PaeR7I  (211)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (211)
1 site
C T C G A G G A G C T C
BmeT110I  (212)
1 site
C Y C G R G G R G C Y C
SacI  (212)
1 site
G A G C T C C T C G A G
BglII  (215)
1 site
A G A T C T T C T A G A
PstI  (223)
1 site
C T G C A G G A C G T C
PvuII  (224)
1 site
C A G C T G G T C G A C
Acc65I  (227)
1 site
G G T A C C C C A T G G
BtgI  (231)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
KpnI  (231)
1 site
G G T A C C C C A T G G
NcoI  (231)
1 site
C C A T G G G G T A C C
EcoRI  (236)
1 site
G A A T T C C T T A A G
BstBI  (240)
1 site
T T C G A A A A G C T T
HindIII  (243)
1 site
A A G C T T T T C G A A
BlpI  (301)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (328)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BspEI  (378)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
NgoMIV  (581)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
NaeI  (583)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BtgZI  (681)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaAI  (686)
1 site
Y A C G T R R T G C A Y
DraIII  (689)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (814)
1 site
T T A T A A A A T A T T
XmnI  (1229)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaHI  (1289)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
TatI  (1346)
1 site
W G T A C W W C A T G W
ScaI  (1348)
1 site
A G T A C T T C A T G A
AmpR
1042 .. 1902  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1042 .. 1110  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1042 .. 1902  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1111 .. 1902  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1042 .. 1902  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
2073 .. 2661  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2073 .. 2661  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
456 .. 911  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
456 .. 911  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
AmpR promoter
937 .. 1041  =  105 bp
AmpR promoter
937 .. 1041  =  105 bp
T7 terminator
312 .. 359  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
312 .. 359  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
MCS
202 .. 248  =  47 bp
multiple cloning site
MCS
202 .. 248  =  47 bp
multiple cloning site
T7 tag (gene 10 leader)
133 .. 165  =  33 bp
11 amino acids  =  1.1 kDa
Product: leader peptide from bacteriophage T7 gene
10
promotes efficient translation in E. coli
T7 tag (gene 10 leader)
133 .. 165  =  33 bp
11 amino acids  =  1.1 kDa
Product: leader peptide from bacteriophage T7 gene
10
promotes efficient translation in E. coli
Xpress™ tag
169 .. 192  =  24 bp
8 amino acids  =  998.0 Da
   Segment 1:  
   169 .. 177  =  9 bp
   3 amino acids  =  409.5 Da
Product: Xpress™ epitope tag, including an
enterokinase recognition and cleavage site
Xpress™ tag
169 .. 192  =  24 bp
8 amino acids  =  998.0 Da
   Segment 2:  enterokinase site  
   178 .. 192  =  15 bp
   5 amino acids  =  606.5 Da
Product: Xpress™ epitope tag, including an
enterokinase recognition and cleavage site
Xpress™ tag
169 .. 192  =  24 bp
8 amino acids  =  998.0 Da
2 segments
Product: Xpress™ epitope tag, including an
enterokinase recognition and cleavage site
T7 promoter
20 .. 38  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
20 .. 38  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
112 .. 129  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
112 .. 129  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
RBS
84 .. 90  =  7 bp
ribosome binding site
RBS
84 .. 90  =  7 bp
ribosome binding site
ATG
100 .. 102  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
100 .. 102  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter