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Plasmid Files

pSC101

Low copy number plasmid from Salmonella. pSC101 was the first plasmid vector used for cloning.

 
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 EcoRI (9261) NsiI (9011) BsrDI (9009) AscI (8738) RsrII (8700) BaeGI - Bme1580I (8490) PaeR7I - PspXI - XhoI (8187) PvuII (7793) SacII (7564) DraIII (7346) SexAI * (7342) MluI (7181) BstXI (7169) BssS α I (7123) PciI (5668) EarI (5607) SpeI (5201) AflII (4910) HindIII (29) BamHI (375) SgrAI (410) SalI (651) PshAI (716) MscI * (1446) AgeI (1687) DrdI (2100) BsrGI (2159) PacI (2288) PflFI - Tth111I (2744) XmnI (2840) HpaI (3097) BsmBI (3522) SspI (4875) pSC101 9263 bp
EcoRI  (9261)
1 site
G A A T T C C T T A A G
NsiI  (9011)
1 site
A T G C A T T A C G T A
BsrDI  (9009)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
AscI  (8738)
1 site
G G C G C G C C C C G C G C G G
RsrII  (8700)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BaeGI  (8490)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (8490)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
PaeR7I  (8187)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (8187)
1 site
V C T C G A G B B G A G C T C V
XhoI  (8187)
1 site
C T C G A G G A G C T C
PvuII  (7793)
1 site
C A G C T G G T C G A C
SacII  (7564)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
DraIII  (7346)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (7342)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MluI  (7181)
1 site
A C G C G T T G C G C A
BstXI  (7169)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BssSαI  (7123)
1 site
C A C G A G G T G C T C
PciI  (5668)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EarI  (5607)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SpeI  (5201)
1 site
A C T A G T T G A T C A
AflII  (4910)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
HindIII  (29)
1 site
A A G C T T T T C G A A
BamHI  (375)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (410)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SalI  (651)
1 site
G T C G A C C A G C T G
PshAI  (716)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
MscI  (1446)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
AgeI  (1687)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
DrdI  (2100)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BsrGI  (2159)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PacI  (2288)
1 site
T T A A T T A A A A T T A A T T
PflFI  (2744)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2744)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
XmnI  (2840)
1 site
G A A N N N N T T C C T T N N N N A A G
HpaI  (3097)
1 site
G T T A A C C A A T T G
BsmBI  (3522)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
SspI  (4875)
1 site
A A T A T T T T A T A A
TcR
86 .. 1276  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
86 .. 1276  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
Rep101
5273 .. 6223  =  951 bp
316 amino acids  =  37.3 kDa
Product: protein needed for replication with the
pSC101 origin
Rep101
5273 .. 6223  =  951 bp
316 amino acids  =  37.3 kDa
Product: protein needed for replication with the
pSC101 origin
pSC101 ori
5003 .. 5225  =  223 bp
low-copy replication origin that requires the Rep101
protein
pSC101 ori
5003 .. 5225  =  223 bp
low-copy replication origin that requires the Rep101
protein
tet promoter
10 .. 38  =  29 bp
   Segment 1:  -35  
   10 .. 15  =  6 bp
promoter for tetracycline efflux protein gene
tet promoter
10 .. 38  =  29 bp
   Segment 2:  
   16 .. 32  =  17 bp
promoter for tetracycline efflux protein gene
tet promoter
10 .. 38  =  29 bp
   Segment 3:  -10  
   33 .. 38  =  6 bp
promoter for tetracycline efflux protein gene
tet promoter
10 .. 38  =  29 bp
3 segments
promoter for tetracycline efflux protein gene
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