pSF-Core

Promoterless backbone SnapFast™ cloning vector, on which all of the other plasmids from Oxford Genetics are based.

Sequence Author: Oxford Genetics

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
PmeI (3615) AhdI (3378) BmrI (3338) BsaI (3312) BpmI (3309) NmeAIII (3231) BsaHI (2838) PmeI (2494) TatI (2456) PpuMI - SanDI (2428) AscI - BssHII (2337) FseI (2191) NaeI (2189) NgoMIV (2187) SwaI (2085) BspHI (1916) AsiSI - PvuI (5) BglII (232) EagI - NotI (274) HindIII (285) Eco53kI (295) BanII - SacI (297) EcoRI (301) KpnI (317) NcoI (321) KpnI (331) EcoRV (337) AbsI - PaeR7I - PspXI - XhoI (344) XbaI (353) BseRI - BsgI (356) BspDI - ClaI (393) BamHI (402) StuI (412) NheI (418) BmtI (422) AanI - PsiI (568) HpaI (588) MfeI (597) T7 terminator PstI - SbfI (934) PacI (1066) SwaI (1192) DrdI (1304) BssSI (1369) BseYI (1500) PspFI (1504) ApaLI (1510) AlwNI (1612) pSF-Core 3734 bp
PmeI  (3615)
2 sites
G T T T A A A C C A A A T T T G
AhdI  (3378)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmrI  (3338)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BsaI  (3312)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (3309)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (3231)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaHI  (2838)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
PmeI  (2494)
2 sites
G T T T A A A C C A A A T T T G
TatI  (2456)
1 site
W G T A C W W C A T G W
PpuMI  (2428)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (2428)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (2337)
1 site
G G C G C G C C C C G C G C G G
BssHII  (2337)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
FseI  (2191)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (2189)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2187)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (2085)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BspHI  (1916)
1 site
T C A T G A A G T A C T
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5)
1 site
C G A T C G G C T A G C
BglII  (232)
1 site
A G A T C T T C T A G A
EagI  (274)
1 site
C G G C C G G C C G G C
NotI  (274)
1 site
G C G G C C G C C G C C G G C G
HindIII  (285)
1 site
A A G C T T T T C G A A
Eco53kI  (295)
1 site
G A G C T C C T C G A G
BanII  (297)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (297)
1 site
G A G C T C C T C G A G
EcoRI  (301)
1 site
G A A T T C C T T A A G
KpnI  (317)
2 sites
G G T A C C C C A T G G
NcoI  (321)
1 site
C C A T G G G G T A C C
KpnI  (331)
2 sites
G G T A C C C C A T G G
EcoRV  (337)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AbsI  (344)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (344)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (344)
1 site
V C T C G A G B B G A G C T C V
XhoI  (344)
1 site
C T C G A G G A G C T C
XbaI  (353)
1 site
T C T A G A A G A T C T
BseRI  (356)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsgI  (356)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (393)
1 site
A T C G A T T A G C T A
ClaI  (393)
1 site
A T C G A T T A G C T A
BamHI  (402)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StuI  (412)
1 site
A G G C C T T C C G G A
NheI  (418)
1 site
G C T A G C C G A T C G
BmtI  (422)
1 site
G C T A G C C G A T C G
AanI  (568)
1 site
T T A T A A A A T A T T
PsiI  (568)
1 site
T T A T A A A A T A T T
HpaI  (588)
1 site
G T T A A C C A A T T G
MfeI  (597)
1 site
C A A T T G G T T A A C
PstI  (934)
1 site
C T G C A G G A C G T C
SbfI  (934)
1 site
C C T G C A G G G G A C G T C C
PacI  (1066)
1 site
T T A A T T A A A A T T A A T T
SwaI  (1192)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
DrdI  (1304)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BssSI  (1369)
1 site
C A C G A G G T G C T C
BseYI  (1500)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (1504)
1 site
C C C A G C G G G T C G
ApaLI  (1510)
1 site
G T G C A C C A C G T G
AlwNI  (1612)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AmpR
2591 .. 3451  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2591 .. 2659  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2591 .. 3451  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2660 .. 3451  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2591 .. 3451  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1257 .. 1845  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1257 .. 1845  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
rrnG terminator
779 .. 915  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
779 .. 915  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
3464 .. 3600  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
3464 .. 3600  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
SV40 poly(A) signal
467 .. 588  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
467 .. 588  =  122 bp
SV40 polyadenylation signal
MCS
273 .. 358  =  86 bp
multiple cloning site
MCS
273 .. 358  =  86 bp
multiple cloning site
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
960 .. 1031  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
960 .. 1031  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
T7 terminator
703 .. 749  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
703 .. 749  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
stop codons
377 .. 387  =  11 bp
stop codons in all three reading frames
stop codons
377 .. 387  =  11 bp
stop codons in all three reading frames
stop codons
425 .. 435  =  11 bp
stop codons in all three reading frames
stop codons
425 .. 435  =  11 bp
stop codons in all three reading frames
RBS
2578 .. 2583  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
2578 .. 2583  =  6 bp
Shine-Dalgarno ribosome binding site
Kozak sequence
319 .. 325  =  7 bp
Kozak sequence
319 .. 325  =  7 bp
RBS
309 .. 314  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
309 .. 314  =  6 bp
Shine-Dalgarno ribosome binding site
ORF:  2591 .. 3451  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3482 .. 59  =  312 bp
ORF:  103 amino acids  =  11.6 kDa
ORF:  804 .. 1235  =  432 bp
ORF:  143 amino acids  =  16.3 kDa
ORF:  3055 .. 3321  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
Click here to try SnapGene

Download pSF-Core.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.