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Plasmid Files

pSMART® BAC v2.0 (BamHI linearized)

BamHI-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic inserts.

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pSMART BAC v2.0 (BamHI linearized) Sequence and MappSMART BAC v2.0 (BamHI linearized).dna
Map and Sequence File   
Sequence Author:  Lucigen
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 < BamHI > (7620) HindIII (7613) EcoRI (7606) PmlI (7602) AhdI (7594) NotI (7582) SL1 (7534 .. 7554) < BamHI > (0) HindIII (7) EcoRI (14) PmlI (21) AhdI (29) NotI (36) SR4 (72 .. 92) pSMART® BAC v2.0 7620 bp
End  (7620)
0 sites
HindIII  (7613)
2 sites
A A G C T T T T C G A A
EcoRI  (7606)
2 sites
G A A T T C C T T A A G
PmlI  (7602)
2 sites
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (7594)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (7582)
2 sites
G C G G C C G C C G C C G G C G
Start  (0)
0 sites
HindIII  (7)
2 sites
A A G C T T T T C G A A
EcoRI  (14)
2 sites
G A A T T C C T T A A G
PmlI  (21)
2 sites
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (29)
2 sites
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (36)
2 sites
G C G G C C G C C G C C G G C G
SL1
21-mer  /  57% GC
1 binding site
7534 .. 7554  =  21 annealed bases
Tm  =  59°C
SR4
21-mer  /  33% GC
1 binding site
72 .. 92  =  21 annealed bases
Tm  =  52°C
sopA
3199 .. 4374  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
sopA
3199 .. 4374  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F
plasmid
repE
4953 .. 5708  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
repE
4953 .. 5708  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the
bacterial F plasmid
CmR
242 .. 901  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
242 .. 901  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6094 .. 6708  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6094 .. 6708  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1682 .. 2155  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
sopC
1682 .. 2155  =  474 bp
centromere-like partitioning region of the bacterial F
plasmid
cos
1023 .. 1422  =  400 bp
lambda cos site; allows packaging into phage
lambda particles
cos
1023 .. 1422  =  400 bp
lambda cos site; allows packaging into phage
lambda particles
incC
4700 .. 4950  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4700 .. 4950  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5799 .. 6018  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
ori2
5799 .. 6018  =  220 bp
secondary origin of replication for the bacterial F
plasmid; also known as oriS
cat promoter
139 .. 241  =  103 bp
promoter of the E. coli cat gene
cat promoter
139 .. 241  =  103 bp
promoter of the E. coli cat gene
rrnBT1 terminator
90 .. 135  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnBT1 terminator
90 .. 135  =  46 bp
transcription terminator T1 from the E. coli rrnB
gene
loxP
972 .. 1005  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
972 .. 1005  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
tonB terminator
915 .. 946  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
tonB terminator
915 .. 946  =  32 bp
bidirectional E. coli tonB-P14 transcription
terminator
T7Te terminator
7475 .. 7502  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7475 .. 7502  =  28 bp
phage T7 early transcription terminator
T7 promoter
49 .. 67  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
49 .. 67  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7561 .. 7579  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7561 .. 7579  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2228 .. 3199  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
sopB
2228 .. 3199  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F
plasmid
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