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pSpark® Done (linearized)

Linearized high copy vector for very efficient cloning of blunt-ended PCR products, with an MCS containing EcoRI and NotI sites on each side of the insertion site.

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pSpark Done (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Canvax Biotech
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< EcoRV > (3016) EcoRI (3008) SacII (3005) NotI (2999) NcoI - StyI (2993) SphI (2982) AatII (2976) ZraI (2974) ApaI (2970) PspOMI (2966) NaeI (2648) NgoMIV (2646) BtgZI (2546) BsaAI - DraIII (2545) PsiI (2417) XmnI (1950) ScaI (1831) TatI (1829) < EcoRV > (0) SpeI (5) EcoRI (11) BfuAI - BspMI - NotI (18) PstI - SbfI (29) SalI (31) AccI (32) HincII (33) NdeI (38) Eco53kI (48) SacI (50) MluI (55) BstXI (59) NsiI (68) BspQI - SapI (342) PciI (458) BseYI (762) PspFI (766) AlwNI (874) AhdI (1351) BsaI (1412) BpmI (1421) NmeAIII (1499) pSpark® Done 3016 bp
End  (3016)
0 sites
EcoRI  (3008)
2 sites
G A A T T C C T T A A G
SacII  (3005)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
NotI  (2999)
2 sites
G C G G C C G C C G C C G G C G
NcoI  (2993)
1 site
C C A T G G G G T A C C
StyI  (2993)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
SphI  (2982)
1 site
G C A T G C C G T A C G
AatII  (2976)
1 site
G A C G T C