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pSpark® II (linearized)

Linearized high copy vector with a classical MCS for very efficient cloning of blunt-ended PCR products.

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pSpark II (linearized).dna
Map and Sequence File:    Download    Open   
Sequence Author:  Canvax Biotech
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PspOMI (2960) NaeI (2642) NgoMIV (2640) BtgZI (2540) BsaAI - DraIII (2539) PsiI (2411) XmnI (1944) ScaI (1825) TatI (1823) NmeAIII (1493) BpmI (1415) BsaI (1406) ApaI (2964) ZraI (2968) AatII (2970) SphI (2976) NcoI - StyI (2987) SfiI (2989) SacII (2996) < EcoRV > (3001) < EcoRV > (0) SpeI (5) BfuAI - BspMI - NotI (12) PstI - SbfI (23) SalI (25) AccI (26) HincII (27) NdeI (32) Eco53kI (42) SacI (44) MluI (49) BstXI (53) NsiI (62) BspQI - SapI (336) PciI (452) BseYI (756) PspFI (760) AlwNI (868) AhdI (1345) pSpark® II 3001 bp
PspOMI  (2960)
1 site
G G G C C C C C C G G G
NaeI  (2642)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2640)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BtgZI  (2540)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaAI  (2539)
1 site
Y A C G T R R T G C A Y
DraIII  (2539)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (2411)
1 site
T T A T A A A A T A T T
XmnI  (1944)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (1825)
1 site
A G T A C T T C A T G A
TatI  (1823)
1 site
W G T A C W W C A T G W
NmeAIII  (1493)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (1415)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (1406)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.