Sticky ends from different BspQI sites may not be compatible.
SapI (2879) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NspI (2766) 1 site
RCATGYYGTACR
PciI (2762) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
PspFI (2462) 1 site
CCCAGCGGGTCG
BseYI (2458) 1 site
CCCAGCGGGTCG
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI (2353) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
AhdI (1874) 1 site
GACNNNNNGTCCTGNNNNNCAG
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
BsaI (1808) 1 site
GGTCTCNCCAGAGN(N)4
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C.
BpmI (1805) 1 site
CTGGAG(N)14NNGACCTC(N)14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
NmeAIII (1727) 1 site
GCCGAG(N)18-19NNCGGCTC(N)18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
MscI (94) 1 site
TGGCCAACCGGT
BtgI (98) 1 site
CCRYGGGGYRCC
Sticky ends from different BtgI sites may not be compatible.
NcoI (98) 1 site
CCATGGGGTACC
BstBI (134) 1 site
TTCGAAAAGCTT
Acc65I (157) 1 site
GGTACCCCATGG
AgeI (160) 1 site
ACCGGTTGGCCA
KpnI (161) 1 site
GGTACCCCATGG
BseRI (189) 1 site
GAGGAG(N)8NNCTCCTC(N)8
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA.
TspMI (189) 1 site
CCCGGGGGGCCC
XmaI (189) 1 site
CCCGGGGGGCCC
Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI (191) 1 site
CCCGGGGGGCCC
SmaI can be used at 37°C for brief incubations.
SrfI (191) 1 site
GCCCGGGCCGGGCCCG
PstI (212) 1 site
CTGCAGGACGTC
MluI (214) 1 site
ACGCGTTGCGCA
SnaBI (222) 1 site
TACGTAATGCAT
BamHI (228) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoNI (236) 1 site
CCTNNNNNAGGGGANNNNNTCC
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible.
EcoRI (253) 1 site
GAATTCCTTAAG
EcoRV (262) 1 site
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
SalI (268) 1 site
GTCGACCAGCTG
AccI (269) 1 site
GTMKACCAKMTG
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible.
HincII (270) 1 site
GTYRACCARYTG
HindIII (274) 1 site
AAGCTTTTCGAA
PaeR7I (280) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI (280) 1 site
CTCGAGGAGCTC
AvrII (286) 1 site
CCTAGGGGATCC
NheI (291) 1 site
GCTAGCCGATCG
BmtI (295) 1 site
GCTAGCCGATCG
XbaI (297) 1 site
TCTAGAAGATCT
AleI (308) 1 site
CACNNNNGTGGTGNNNNCAC
PmlI (308) 1 site
CACGTGGTGCAC
PmlI gradually loses activity when stored at -20°C.
BstXI (310) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
EcoO109I (315) 1 site
RGGNCCYYCCNGGR
Sticky ends from different EcoO109I sites may not be compatible.