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pTrcHis2 B

Bacterial vector for inducible expression of C-terminally 6xHis-tagged proteins. For other reading frames, use pTrcHis2 A or pTrcHis2 C.

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pTrcHis2 B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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PluTI (4276) SfoI (4274) NarI * (4273) KasI (4272) EcoRV (4083) ApaI (3844) PspOMI (3840) BstEII (3814) BclI * (3647) MluI (3633) PflMI (3215) NsiI (3196) SphI (3194) MauBI (3172) PfoI (3104) PflFI - Tth111I (3005) BsaAI (2999) BstZ17I (2980) BspQI - SapI (2866) PciI (2749) AlwNI (2340) BfuAI - BspMI (72) BtgI - NcoI - StyI (411) BamHI (415) Eco53kI (423) AvaI - BsoBI - PaeR7I - XhoI (424) BmeT110I - SacI (425) BglII (428) PstI (436) Acc65I (440) KpnI (444) EcoRI (452) BstBI (456) HindIII (459) XbaI (465) SalI (508) PmeI (538) ScaI (1380) TsoI (1463) PvuI (1492) FspI (1638) BglI (1743) AhdI (1861) pTrcHis2 B 4404 bp
PluTI  (4276)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4274)
1 site
G G C G C C C C G C G G
NarI  (4273)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4272)
1 site
G G C G C C C C G C G G