Resources
Plasmid Files

pTrcHis2 C

Bacterial vector for inducible expression of C-terminally 6xHis-tagged proteins. For other reading frames, use pTrcHis2 A or pTrcHis2 B.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pTrcHis2 C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
Download Free Trial Get SnapGene Viewer


PluTI (4277) SfoI (4275) NarI * (4274) KasI (4273) EcoRV (4084) ApaI (3845) PspOMI (3841) BstEII (3815) BclI * (3648) MluI (3634) PflMI (3216) NsiI (3197) SphI (3195) MauBI (3173) PfoI (3105) PflFI - Tth111I (3006) BstZ17I (2981) BspQI - SapI (2867) PciI (2750) AlwNI (2341) BfuAI - BspMI (72) BtgI - NcoI - StyI (411) BamHI (415) Eco53kI (423) AvaI - BsoBI - PaeR7I - XhoI (424) BmeT110I - SacI (425) BglII (428) PstI (436) Acc65I (440) KpnI (444) EcoRI (452) BstBI (456) HindIII (459) SnaBI (466) SalI (509) PmeI (539) ScaI (1381) TsoI (1464) PvuI (1493) FspI (1639) BglI (1744) AhdI (1862) pTrcHis2 C 4405 bp
PluTI  (4277)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4275)
1 site
G G C G C C C C G C G G
NarI  (4274)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4273)
1 site
G G C G C C C C G C G G
EcoRV  (4084)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (3845)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3841)
1 site
G G G C C C C C C G G G
BstEII  (3815)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BclI  (3648)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MluI  (3634)
1 site
A C G C G T T G C G C A
PflMI  (3216)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NsiI  (3197)
1 site
A T G C A T T A C G T A
SphI  (3195)
1 site
G C A T G C