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pTrcHis A

Bacterial vector for inducible expression of N-terminally 6xHis-tagged proteins. For other reading frames, use pTrcHis B or pTrcHis C.

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pTrcHis A.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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PluTI (4286) SfoI (4284) NarI * (4283) KasI (4282) EcoRV (4093) ApaI (3854) PspOMI (3850) BstEII (3824) BclI * (3657) MluI (3643) PflMI (3225) NsiI (3206) SphI (3204) MauBI (3182) PfoI (3114) PflFI - Tth111I (3015) BsaAI (3009) BstZ17I (2990) AccI (2989) BspQI - SapI (2876) PciI (2759) AlwNI (2350) BfuAI - BspMI (72) BtgI - NcoI - StyI (411) NheI (449) BmtI (453) BamHI (515) Eco53kI (523) AvaI - BsoBI - PaeR7I - XhoI (524) BmeT110I - SacI (525) BglII (528) PstI (536) Acc65I (540) KpnI (544) EcoRI (552) BstBI (556) HindIII (559) ScaI (1390) PvuI (1502) FspI (1648) BglI (1753) AhdI (1871) pTrcHis A 4414 bp
PluTI  (4286)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4284)
1 site
G G C G C C C C G C G G
NarI  (4283)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4282)
1 site
G G C G C C C C G C G G
EcoRV  (4093)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (3854)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3850)
1 site
G G G C C C C C C G G G
BstEII  (3824)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BclI  (3657)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MluI  (3643)
1 site
A C G C G T T G C G C A
PflMI  (3225)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NsiI  (3206)
1 site
A T G C A T T A C G T A
SphI  (3204)
1 site
G C A T G C C G T A C G
MauBI  (3182)
1 site
C G C G C G C G G C G C G C G C
PfoI  (3114)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (3015)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3015)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (3009)
1 site
Y A C G T R R T G C A Y
BstZ17I  (2990)
1 site
G T A T A C C A T A T G
AccI  (2989)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BspQI  (2876)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2876)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (2759)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (2350)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BfuAI  (72)
1 site
A C C T G C ( N ) 4 T