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Plasmid Files

pUC119

Cloning vector with a phage origin for producing single-stranded DNA. The MCS is reversed in pUC118.

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pUC119 Sequence and MappUC119.dna
Map and Sequence File   
Sequence Author:  TaKaRa
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 EcoO109I (3151) AatII (3097) ZraI (3095) XmnI (2774) ScaI (2655) NmeAIII (2323) BpmI (2245) BsaI (2236) AhdI (2175) AlwNI (1698) PspFI (1590) PfoI (46) PsiI (283) BsaAI - DraIII (411) BtgZI (412) NgoMIV (512) NaeI (514) Bpu10I (562) KasI (711) NarI (712) SfoI (713) PluTI (715) M13 fwd EcoRI (872) Eco53kI (880) SacI (882) Acc65I (884) KpnI - TspMI - XmaI (888) SmaI (890) BamHI (893) XbaI (899) SalI (905) AccI (906) HincII (907) PstI - SbfI (915) BfuAI - BspMI (918) SphI (921) HindIII (923) lac operator BspQI - SapI (1166) AflIII - PciI (1282) BseYI (1586) pUC119 3162 bp
EcoO109I  (3151)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3097)
1 site
G A C G T C C T G C A G
ZraI  (3095)
1 site
G A C G T C C T G C A G
XmnI  (2774)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (2655)
1 site
A G T A C T T C A T G A
NmeAIII  (2323)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (2245)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (2236)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2175)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1698)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1590)
1 site
C C C A G C G G G T C G
PfoI  (46)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PsiI  (283)
1 site
T T A T A A A A T A T T
BsaAI  (411)
1 site
Y A C G T R R T G C A Y
DraIII  (411)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BtgZI  (412)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
NgoMIV  (512)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
NaeI  (514)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
Bpu10I  (562)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
KasI  (711)
1 site
G G C G C C C C G C G G
NarI  (712)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (713)
1 site
G G C G C C C C G C G G
PluTI  (715)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
EcoRI  (872)
1 site
G A A T T C C T T A A G
Eco53kI  (880)
1 site
G A G C T C C T C G A G
SacI  (882)
1 site
G A G C T C C T C G A G
Acc65I  (884)
1 site
G G T A C C C C A T G G
KpnI  (888)
1 site
G G T A C C C C A T G G
TspMI  (888)
1 site
C C C G G G G G G C C C
XmaI  (888)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (890)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (893)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
XbaI  (899)
1 site
T C T A G A A G A T C T
SalI  (905)
1 site
G T C G A C C A G C T G
AccI  (906)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (907)
1 site
G T Y R A C C A R Y T G
PstI  (915)
1 site
C T G C A G G A C G T C
SbfI  (915)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (918)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (918)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (921)
1 site
G C A T G C C G T A C G
HindIII  (923)
1 site
A A G C T T T T C G A A
BspQI  (1166)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1166)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
AflIII  (1282)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (1282)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BseYI  (1586)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
AmpR
2102 .. 2962  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   2102 .. 2893  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2102 .. 2962  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2894 .. 2962  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2102 .. 2962  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
1343 .. 1931  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1343 .. 1931  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
M13 ori
187 .. 642  =  456 bp
M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
M13 ori
187 .. 642  =  456 bp
M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
AmpR promoter
2963 .. 3067  =  105 bp
AmpR promoter
2963 .. 3067  =  105 bp
MCS
872 .. 928  =  57 bp
pUC18/19 multiple cloning site
MCS
872 .. 928  =  57 bp
pUC18/19 multiple cloning site
lac promoter
989 .. 1019  =  31 bp
   Segment 3:  -10  
   989 .. 995  =  7 bp
promoter for the E. coli lac operon
lac promoter
989 .. 1019  =  31 bp
   Segment 2:  
   996 .. 1013  =  18 bp
promoter for the E. coli lac operon
lac promoter
989 .. 1019  =  31 bp
   Segment 1:  -35  
   1014 .. 1019  =  6 bp
promoter for the E. coli lac operon
lac promoter
989 .. 1019  =  31 bp
3 segments
promoter for the E. coli lac operon
M13 fwd
855 .. 871  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
855 .. 871  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
941 .. 957  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
941 .. 957  =  17 bp
common sequencing primer, one of multiple similar
variants
lac operator
965 .. 981  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
965 .. 981  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lacZα
619 .. 945  =  327 bp
108 amino acids  =  12.4 kDa
Product: LacZα fragment of β-galactosidase
lacZα
619 .. 945  =  327 bp
108 amino acids  =  12.4 kDa
Product: LacZα fragment of β-galactosidase
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