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pVLT35

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a spectinomycin/streptomycin resistance gene.

 
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pVLT35.dna
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SalI (9527) XbaI (9521) BamHI (9515) SmaI (9512) KpnI - TspMI - XmaI (9510) Acc65I (9506) SacI (9504) Eco53kI (9502) EcoRI (9494) MauBI (9219) AclI (9102) MluI (8762) ApaI (8559) PspOMI (8555) HpaI (8260) BsmBI (8147) BspQI - SapI (7954) FspAI (7483) EcoNI (7333) PfoI * (7193) SgrAI (5245) PstI - SbfI (9537) BsaBI * (224) XmnI (338) EagI (377) BsaI (468) PvuI (1231) NgoMIV (1499) NaeI (1501) SfiI (2385) BstZ17I (3760) PasI (4766) pVLT35 9575 bp
SalI  (9527)
1 site
G T C G A C C A G C T G
XbaI  (9521)
1 site
T C T A G A A G A T C T
BamHI  (9515)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (9512)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (9510)
1 site
G G T A C C C C A T G G
TspMI  (9510)
1 site
C C C G G G G G G C C C
XmaI  (9510)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
Acc65I  (9506)
1 site
G G T A C C C C A T G G
SacI  (9504)
1 site
G A G C T C C T C G A G
Eco53kI  (9502)
1 site
G A G C T C C T C G A G
EcoRI  (9494)
1 site
G A A T T C C T T A A G
MauBI  (9219)
1 site
C G C G C G C G G C G C G C G C
AclI  (9102)
1 site
A A C G T T T T G C A A
MluI  (8762)
1 site
A C G C G T T G C G C A
ApaI  (8559)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (8555)
1 site
G G G C C C C C C G G G
HpaI  (8260)
1 site
G T T A A C C A A T T G
BsmBI  (8147)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BspQI  (7954)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7954)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (7483)
1 site
R T G C G C A Y Y A C G C G T R
EcoNI  (7333)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PfoI  (7193)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SgrAI  (5245)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
PstI  (9537)
1 site
C T G C A G G A C G T C
SbfI  (9537)
1 site
C C T G C A G G G G A C G T C C
BsaBI  (224)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
XmnI  (338)
1 site
G A A N N N N T T C C T T N N N N A A G
EagI  (377)
1 site
C G G C C G G C C G G C
BsaI  (468)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PvuI  (1231)
1 site
C G A T C G G C T A G C
NgoMIV  (1499)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1501)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
SfiI  (2385)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from differe