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Plasmid Files

Cas9m4

Plasmid for expression in mammalian cells of catalytically dead Cas9m4.

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Cas9m4.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Church Lab / Addgene #47316
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AhdI (8540) SgrDI (7614) MfeI (7451) MluI (7384) SpeI (7363) SnaBI (7026) XbaI (6704) SbfI (6440) XcmI (6333) BlpI (5732) Bsu36I (5414) BstZ17I (258) RsrII (713) AvrII (1438) StuI (1443) SexAI * (1670) DraIII (1968) PmeI (2510) AgeI (2524) AscI (2724) EcoRI (2857) PaeR7I - XhoI (3136) AarI (3419) FspAI (3733) PmlI (3894) BstXI (3898) BmgBI (4173) BamHI (4674) Cas9m4 9553 bp
AhdI  (8540)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SgrDI  (7614)
1 site
C G T C G A C G G C A G C T G C
MfeI  (7451)
1 site
C A A T T G G T T A A C
MluI  (7384)
1 site
A C G C G T T G C G