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Plasmid Files

MLM3613

Plasmid for expressing S. pyogenes Cas9 from either the CMV promoter in vivo or the T7 promoter in vitro.

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MLM3613 Sequence and MapMLM3613.dna
Map and Sequence File   
Sequence Author:  Joung Lab / Addgene #42251
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 PmeI (4593) MLM3613 7554 bp
PmeI  (4593)
1 site
G T T T A A A C C A A A T T T G
Cas9
409 .. 4512  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
409 .. 4512  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
AmpR
6071 .. 6931  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6071 .. 6862  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6071 .. 6931  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6863 .. 6931  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6071 .. 6931  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
5312 .. 5900  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5312 .. 5900  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
7306 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7306 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
4615 .. 4839  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4615 .. 4839  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
AmpR promoter
6932 .. 7036  =  105 bp
AmpR promoter
6932 .. 7036  =  105 bp
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
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