MSP2133

Mammalian cell plasmid for expressing the high-fidelity S. pyogenes Cas9 variant SpCas9-HF4.

Sequence Author: Joung Lab / Addgene #72249

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SV40 NLS MSP2133 7614 bp
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 1:  
   409 .. 1755  =  1347 bp
   449 amino acids  =  51.8 kDa
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 2:  
   1756 .. 1758  =  3 bp
   1 amino acid  =  89.1 Da
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 3:  
   1759 .. 1896  =  138 bp
   46 amino acids  =  5.3 kDa
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 4:  
   1897 .. 1899  =  3 bp
   1 amino acid  =  89.1 Da
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 5:  
   1900 .. 2388  =  489 bp
   163 amino acids  =  19.4 kDa
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 6:  
   2389 .. 2391  =  3 bp
   1 amino acid  =  89.1 Da
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 7:  
   2392 .. 2490  =  99 bp
   33 amino acids  =  3.8 kDa
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 8:  
   2491 .. 2493  =  3 bp
   1 amino acid  =  89.1 Da
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 9:  
   2494 .. 3183  =  690 bp
   230 amino acids  =  26.5 kDa
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 10:  
   3184 .. 3186  =  3 bp
   1 amino acid  =  89.1 Da
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
   Segment 11:  
   3187 .. 4512  =  1326 bp
   442 amino acids  =  51.0 kDa
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SpCas9-HF4
409 .. 4512  =  4104 bp
1368 amino acids  =  158.1 kDa
11 segments
Product: Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system, mutated to improve targeting specificity (Kleinstiver et al., 2016)
carries the mutations Y450A, N497A, R661A, Q695A, and Q926A
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4519 .. 4539  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
4546 .. 4611  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6131 .. 6922  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6923 .. 6991  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6131 .. 6991  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5372 .. 5960  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
7366 .. 131  =  380 bp
human cytomegalovirus immediate early enhancer
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4675 .. 4899  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
132 .. 335  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
6992 .. 7096  =  105 bp
AmpR promoter
6992 .. 7096  =  105 bp
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
377 .. 395  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  409 .. 4623  =  4215 bp
ORF:  1404 amino acids  =  162.2 kDa
ORF:  6261 .. 6527  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2373 .. 2660  =  288 bp
ORF:  95 amino acids  =  10.5 kDa
ORF:  3522 .. 3767  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  6131 .. 6991  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Download MSP2133.dna file

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Individual Sequences & Maps

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