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Plasmid Files

MSP2135

Mammalian cell plasmid for expressing the high-fidelity S. pyogenes Cas9 variant SpCas9-HF2.

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MSP2135.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Joung Lab / Addgene #72248
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AhlI - BcuI - SpeI (7380) MluI (7359) SgrDI (7125) Acc113I - BmcAI - ScaI - ZrmI (6684) BpvUI - Ple19I - PvuI (6574) BsaI - Bso31I - BspTNI - Eco31I (6265) AhdI - AspEI - BmeRI - DriI - Eam1105I (6204) BspQI - LguI - PciSI - SapI (5195) BbsI - BpiI - BpuAI - BstV2I (4864) MssI - PmeI (4653) AgeI - AsiGI - BshTI - CspAI - PinAI (4623) PstI (4617) AanI - PsiI (4571) PaeR7I - Sfr274I - SlaI - XhoI (4539) BamHI (4513) BmtI - BspOI (4055) AsuNHI - NheI (4051) BseX3I - BstZI - CciNI - Cfr42I - EagI - EclXI - Eco52I - KspI - NotI - SacII - Sfr303I - SgrBI - SstII (370) BssNAI - Bst1107I - BstZ17I (623) AjiI - BmgBI - BtrI (628) BalI - MlsI - MluNI - MscI - Msp20I (679) AxyI - Bse21I - Bsu36I - Eco81I (859) BstEII - BstPI - Eco91I - EcoO65I - PspEI (1123) AatI - Eco147I - PceI - SseBI - StuI (1148) BsiWI - Pfl23II - PspLI (1217) KasI - SspDI (1350) Mly113I - NarI (1351) DinI - EgeI - EheI - SfoI (1352) BbeI - PluTI (1354) Acc36I - BfuAI - BspMI - BveI (1477) Bsp1407I - BsrGI - BstAUI - SspBI (1967) PfoI * (2225) Eco32I - EcoRV (2250) FspAI (3359) BsgI (3727)