PX551

Zhang lab AAV vector, also known as AAV-SpCas9 or pAAV-SpCas9, for expressing Cas9 in neurons. Use together with PX552.

Sequence Author: Zhang Lab / Addgene #60957

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ATG SV40 NLS PX551 7400 bp
ATG
401 .. 403  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
401 .. 403  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
HA
404 .. 430  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
404 .. 430  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
SV40 NLS
434 .. 454  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
434 .. 454  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
464 .. 4564  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
464 .. 4564  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4568 .. 4588  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4568 .. 4588  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
5720 .. 6580  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5720 .. 5788  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5720 .. 6580  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5789 .. 6580  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5720 .. 6580  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
6751 .. 7339  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6751 .. 7339  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4878 .. 5333  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4878 .. 5333  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
Mecp2 promoter
160 .. 388  =  229 bp
promoter for selective expression in neurons (Adachi et al., 2005; Gray et al., 2011)
Mecp2 promoter
160 .. 388  =  229 bp
promoter for selective expression in neurons (Adachi et al., 2005; Gray et al., 2011)
AAV2 ITR
4663 .. 4803  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
4663 .. 4803  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
5615 .. 5719  =  105 bp
AmpR promoter
5615 .. 5719  =  105 bp
poly(A) signal
4607 .. 4655  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4607 .. 4655  =  49 bp
synthetic polyadenylation signal
ORF:  401 .. 4600  =  4200 bp
ORF:  1399 amino acids  =  162.0 kDa
ORF:  5720 .. 6580  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  264 .. 521  =  258 bp
ORF:  85 amino acids  =  9.3 kDa
ORF:  4677 .. 4901  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  84 .. 410  =  327 bp
ORF:  108 amino acids  =  11.5 kDa
ORF:  230 .. 2980  =  2751 bp
ORF:  916 amino acids  =  96.4 kDa
ORF:  4628 .. 4858  =  231 bp
ORF:  76 amino acids  =  8.8 kDa
ORF:  1810 .. 2103  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  2374 .. 2823  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  6184 .. 6450  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download PX551.dna file

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