pAAV-Guide-it-Up Vector

CRISPR/Cas9 adeno-associated virus (AAV) vector for cloning and expression of a single guide RNA (sgRNA). Use together with pAAV-Guide-it-Down Vector.

Sequence Author: Clontech (TaKaRa)

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pAAV-Guide-it-Up Vector 7117 bp
Cas9-Up
748 .. 3853  =  3106 bp
1035 amino acids  =  120.3 kDa
Cas9-Up
748 .. 3853  =  3106 bp
1035 amino acids  =  120.3 kDa
AmpR
5081 .. 5941  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5081 .. 5149  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5081 .. 5941  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5150 .. 5941  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5081 .. 5941  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
6112 .. 6700  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6112 .. 6700  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4239 .. 4694  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4239 .. 4694  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
210 .. 513  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
210 .. 513  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
514 .. 717  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
514 .. 717  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
3860 .. 4000  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
3860 .. 4000  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
4976 .. 5080  =  105 bp
AmpR promoter
4976 .. 5080  =  105 bp
ORF:  673 .. 3855  =  3183 bp
ORF:  1060 amino acids  =  123.1 kDa
ORF:  3874 .. 4155  =  282 bp
ORF:  93 amino acids  =  9.9 kDa
ORF:  5081 .. 5941  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3911 .. 4219  =  309 bp
ORF:  102 amino acids  =  11.4 kDa
ORF:  5545 .. 5811  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2097 .. 2390  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  2712 .. 3110  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
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Download pAAV-Guide-it-Up Vector.dna file

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