Resources
Plasmid Files

pAC155-pCR8-sgExpression

Gateway® entry vector for expression of a single guide RNA (sgRNA) from the U6 promoter.

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pAC155-pCR8-sgExpression Sequence and MappAC155-pCR8-sgExpression.dna
Map and Sequence File   
Sequence Author:  Jaenisch Lab / Addgene #49045
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 polIII terminator BbsI (775) BbsI (797) pAC155-pCR8-sgExpression 3178 bp
BbsI  (775)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (797)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SmR
1644 .. 2435  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase
(Murphy, 1985)
confers resistance to spectinomycin and
streptomycin
SmR
1644 .. 2435  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase
(Murphy, 1985)
confers resistance to spectinomycin and
streptomycin
ori
2528 .. 3116  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2528 .. 3116  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
U6 promoter
806 .. 1046  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
806 .. 1046  =  241 bp
RNA polymerase III promoter for human U6 snRNA
attL1
509 .. 608  =  100 bp
recombination site for the Gateway® LR reaction
attL1
509 .. 608  =  100 bp
recombination site for the Gateway® LR reaction
attL2
1057 .. 1156  =  100 bp
recombination site for the Gateway® LR reaction
attL2
1057 .. 1156  =  100 bp
recombination site for the Gateway® LR reaction
gRNA scaffold
704 .. 779  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
704 .. 779  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
rrnB T2 terminator
268 .. 295  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
rrnB T2 terminator
268 .. 295  =  28 bp
transcription terminator T2 from the E. coli rrnB
gene
M13 fwd
477 .. 493  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
477 .. 493  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
1197 .. 1213  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
1197 .. 1213  =  17 bp
common sequencing primer, one of multiple similar
variants
polIII terminator
698 .. 703  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
698 .. 703  =  6 bp
RNA polymerase III transcription terminator
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