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Plasmid Files

pCAG-T3-hCasD10A-pA

Plasmid for mammalian cell expression or in vitro transcription of human codon-optimized Cas9 with the D10A nickase mutation.

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pCAG-T3-hCasD10A-pA.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Fujii & Naito Labs / Addgene #51638
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BspQI - SapI (9212) AhdI (8207) SpeI (7265) SnaBI (6928) ApaI (6445) PspOMI (6441) BsiWI (6113) MreI - SgrAI (5987) XbaI (5672) AleI (5531) NotI (5520) SrfI (5510) BsmBI (5252) SbfI (5175) XcmI (5068) AvrII (156) StuI (161) SfiI (210) SphI (1092) PspXI (1097) Acc65I (1268) KpnI (1272) BspDI - ClaI (1275) AscI (1459) NruI (2178) FspAI (2468) PmlI (2629) BmgBI (2908) pCAG-T3-hCasD10A-pA 9272 bp