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Plasmid Files

pCLIP-Cas9-Nickase-EFS-Blast

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and a blasticidin resistance marker expressed from the EF-1α core promoter.

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pCLIP-Cas9(D10a)-Nickase-EFS-Blast.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (10,377) FspI (9795) AflIII - PciI (8683) SmaI (8663) TspMI - XmaI (8661) AvrII (8640) SexAI * (8407) PmeI (8315) PsiI (8265) KpnI (7930) Acc65I (7926) Bsu36I (7922) PaeR7I - XhoI (7808) SalI (7802) BspDI - ClaI (7794) SacII (7707) EcoRI (7180) BamHI (6715) PmlI (5328) SpeI (41) NdeI (276) SnaBI (382) KasI (671) NarI (672) SfoI (673) PluTI (675) BssHII (745) BspQI - SapI (1136) NotI (1178) BbvCI (1454) AleI (1607) AgeI (2516) XbaI (2522) AfeI (2529) PspOMI (3894) ApaI (3898) PasI (4308) pCLIP-Cas9-Nickase-EFS-Blast 10,495 bp