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Plasmid Files

pCLIP-Cas9(D10a)-Nickase-EFS-Puro

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and a puromycin resistance marker expressed from the EF-1α core promoter.

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pCLIP-Cas9(D10a)-Nickase-EFS-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (10,575) PvuI (10,141) FspI (9993) DrdI (8989) AflIII - PciI (8881) SmaI (8861) TspMI - XmaI (8859) AvrII (8838) PmeI (8513) PsiI (8463) KpnI (8128) Acc65I (8124) Bsu36I (8120) PaeR7I - XhoI (8006) SalI (8000) BspDI - ClaI (7992) EcoRI (7378) BstEII (6912) RsrII (6894) BsiWI (6834) PflFI - Tth111I (6820) BamHI (6715) BsaBI * (6037) SpeI (41) NdeI (276) SnaBI (382) NruI * (867) BspQI - SapI (1136) NotI (1178) BbvCI (1454) AleI (1607) AgeI (2516) XbaI (2522) AfeI (2529) PspOMI (3894) ApaI (3898) PmlI (5328)