Resources
Plasmid Files

pCLIP-Cas9-Nickase-EFS-ZsGreen

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and the green fluorescent protein ZsGreen expressed from the EF-1α core promoter.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCLIP-Cas9(D10a)-Nickase-EFS-ZsGreen.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
Download Free Trial Get SnapGene Viewer


SspI (10,674) PvuI (10,240) FspI (10,092) DrdI (9088) AflIII - PciI (8980) SmaI (8960) TspMI - XmaI (8958) AvrII (8937) SexAI * (8704) PmeI (8612) PsiI (8562) KpnI (8227) Acc65I (8223) Bsu36I (8219) PaeR7I - XhoI (8105) BspDI - ClaI (8091) SacII (8004) EcoRI (7477) BsrGI (7331) PflFI - Tth111I (7015) SgrAI (6868) BamHI (6715) BsaBI * (6037) SpeI (41) NdeI (276) SnaBI (382) BssHII (745) NruI * (867) BspQI - SapI (1136) NotI (1178) BbvCI (1454) AleI (1607) AgeI (2516) XbaI (2522) AfeI (2529) PspOMI (3894) ApaI (3898) PasI (4308) PmlI (5328) pCLIP-Cas9-Nickase-EFS-ZsGreen 10,792 bp